FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4B10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4B10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1430
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA19213.426573426573427No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA1037.2027972027972025No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG674.685314685314685No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA674.685314685314685No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA634.405594405594406No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT634.405594405594406No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA553.8461538461538463No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA443.076923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG231.6083916083916083No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG191.3286713286713288No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG181.2587412587412588No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG181.2587412587412588No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG151.048951048951049No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG151.048951048951049No Hit
TACCCTTACGATGTTCCTGATTACGCGTAAATTCCTAGTCGAAGATTTCG110.7692307692307693No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC100.6993006993006993No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA100.6993006993006993No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG90.6293706293706294No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG90.6293706293706294No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT90.6293706293706294No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGCTCGAAGATTTCGTTGG90.6293706293706294No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG90.6293706293706294No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG80.5594405594405595No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT70.48951048951048953No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG70.48951048951048953No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA70.48951048951048953No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG70.48951048951048953No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT60.4195804195804196No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG60.4195804195804196No Hit
TACCCTTACGATGTTCCTGATTACGCACAAGAGTTTGCCAGCCCGCCTAA50.34965034965034963No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCGCCTAA50.34965034965034963No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGAAGATTTCGTTGGGGACTCT50.34965034965034963No Hit
TACCCTTACGATGTTCCTGATTACGGTCTTGGGCTCGAAGATTTCGTTGG50.34965034965034963No Hit
TACCCTTACGATGTTCCTGATTACGCAGTGGGCTCGAAGATTTCGTTGGG40.27972027972027974No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCATCCCTCCTAA40.27972027972027974No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT40.27972027972027974No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG40.27972027972027974No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGGCTCGAAGATTTCGTTG40.27972027972027974No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA30.2097902097902098No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGA30.2097902097902098No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG30.2097902097902098No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA30.2097902097902098No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCATCCCGCCTAA30.2097902097902098No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA30.2097902097902098No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTAGTGGGCTCGAAGATTTCGT30.2097902097902098No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGGTTGCCAGCCCGCCTAA30.2097902097902098No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAACAGCCCGCCTAATG30.2097902097902098No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCATCCCTCCTAA30.2097902097902098No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCGCCTAA20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT20.13986013986013987No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.13986013986013987No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT20.13986013986013987No Hit
TACCCTTACTATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCACACAGCCCGCCTAA20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGGTGCTCGAAGATTTCTTTGTGGACT20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGG20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGTTCTCGAATCTTTCGTTT20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCACGATTTGCTGCCAGCCCGCCTAA20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCTCCTAA20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA20.13986013986013987No Hit
TACCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAAT20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCATAGAGCAAATGCAGCCCGCCTAA20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGGTGTGCTCTCAGATTTCGTTGGGGA20.13986013986013987No Hit
TACCCTTACGCTGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCATGAATTGCTGCCAGCCCGCCTAA20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCTCCTAA20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA20.13986013986013987No Hit
TACCCTTACGATGTTCCTGCTTACGCATGATTTGCTGCCAGCCCGCCTAA20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCTCCTGGGCTCGAAGATTTCGTTGG20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCATCCCGCCTAA20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGCTTTCGTTG20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGTCCTCT20.13986013986013987No Hit
TACCCTTACGATGTTCCTGCTTACGCATAAGAGTTTGCCAGCCCGCCTAA20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGGTCGAAGATTTCGTTGGGGA20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC20.13986013986013987No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGCTCGAAGATTTCGTTGGGGA20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCTCCTAA20.13986013986013987No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGT20.13986013986013987No Hit
TACCCTTACGCTGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA20.13986013986013987No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA20.13986013986013987No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)