FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4B06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4B06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1084
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA14012.915129151291513No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG1029.40959409594096No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT756.918819188191883No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG484.428044280442804No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA403.6900369003690034No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG292.6752767527675276No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG252.3062730627306274No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA252.3062730627306274No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG232.121771217712177No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA222.029520295202952No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA181.6605166051660518No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG151.3837638376383763No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG131.1992619926199262No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC111.014760147601476No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG111.014760147601476No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG100.9225092250922509No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG100.9225092250922509No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA90.8302583025830259No Hit
TACCCTTACGATGTTCCTGATTACGCCTGGGGCTCGAAGATTTCGTTGGG90.8302583025830259No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG90.8302583025830259No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT80.7380073800738007No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG80.7380073800738007No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT60.5535055350553505No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG60.5535055350553505No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT60.5535055350553505No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA60.5535055350553505No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGCTCGAAGATTTCGTTGG50.46125461254612543No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA50.46125461254612543No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG50.46125461254612543No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGAAGATTTCGTTGGGGACTC40.36900369003690037No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA40.36900369003690037No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG40.36900369003690037No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA30.27675276752767525No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTCAGATTTCGTTGGGGA30.27675276752767525No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGCTGGGGA30.27675276752767525No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT30.27675276752767525No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT30.27675276752767525No Hit
GATGAGTCAGCGTAGAACATGCAACATTTATTGCATGATACGTGGCATTT30.27675276752767525No Hit
TACCCTTACGCTGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA30.27675276752767525No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCATCCCGCCTAA30.27675276752767525No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA30.27675276752767525No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG30.27675276752767525No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT30.27675276752767525No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT30.27675276752767525No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGCTCGATGATTTCGTTGGGGACTCTG20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGCTTTCGTTG20.18450184501845018No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTTGCATTT20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTGGGGACTCTGT20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTATGGCTCGAAGATTTCGTTG20.18450184501845018No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGATCTGT20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGACTCGAAGATTTCGTTGGGG20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTT20.18450184501845018No Hit
GATGAGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGTTCGAAGATTTCGTTGGGGA20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG20.18450184501845018No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGGATTT20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGGAGATTTCGTTGG20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGAATCTG20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGT20.18450184501845018No Hit
TACCCTTACGATGTTCCTGATTACGCTGAGCTCGAAGATTTCGTTGGGGA20.18450184501845018No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)