FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4B04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4B04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA63332.19735503560529No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA1457.375381485249237No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT1346.8158697863682605No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA904.5778229908443535No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA502.5432349949135302No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG452.2889114954221768No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA452.2889114954221768No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG291.4750762970498474No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG221.119023397761953No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA190.9664292980671414No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA170.8646998982706002No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG150.7629704984740591No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG140.7121057985757884No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG140.7121057985757884No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT130.6612410986775178No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA120.6103763987792472No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG120.6103763987792472No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACAGCCCGCCTAA110.5595116988809765No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG110.5595116988809765No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA90.4577822990844354No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG80.40691759918616477No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCTCCTAA80.40691759918616477No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT80.40691759918616477No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCACACAGCCCGCCTAA70.3560528992878942No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGGGCAAACAGCCCGCCTAA60.3051881993896236No Hit
TACCCTTACGATGTTCCTGATTACGCATAAAGTGCAAACAGCCCGCCTAA60.3051881993896236No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA60.3051881993896236No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG50.254323499491353No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT50.254323499491353No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT50.254323499491353No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCGCCTAA40.20345879959308238No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAATGCAGCCCGCCT40.20345879959308238No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA40.20345879959308238No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT40.20345879959308238No Hit
TACCCTTACTATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA40.20345879959308238No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCGAA40.20345879959308238No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG40.20345879959308238No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGCTCGAAGATTTCGTTGG40.20345879959308238No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCTCCTAA40.20345879959308238No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAATCAGCCCGCCTAA30.1525940996948118No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA30.1525940996948118No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGTCTCGAATATTTCGTTGG30.1525940996948118No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGGTTGCCAGCCCGCCTAA30.1525940996948118No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGCTCGAAGATTTCGTTGGGG30.1525940996948118No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT30.1525940996948118No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCTCCTAA30.1525940996948118No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG30.1525940996948118No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG30.1525940996948118No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT30.1525940996948118No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACGTTCGT30.1525940996948118No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA20.10172939979654119No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACATCCCTCCTAA20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTCAGATTTCGTTGGGGA20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGCTTTCGTTG20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA20.10172939979654119No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCATCCCGCCTAA20.10172939979654119No Hit
TACCCTTACGATGTTCCTGCTTACGCATAATGTTCAAACATCCCTCATAA20.10172939979654119No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT20.10172939979654119No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACCTTCGT20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGATCTGT20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACTGGGCTCGAAGATTTCGTTGGGGACT20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACTCTGGGCTCGAAGATTTCGTTGGGGA20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCTTTGGGGCCTCT20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTTCCAGCCCGCCTAA20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGTGCTCGAAGATTTCGTT20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTTCAAACATCCCGCCTAA20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCAGCCCTCCTAA20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT20.10172939979654119No Hit
TACACTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTTA20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA20.10172939979654119No Hit
TACCCTTACGCTGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG20.10172939979654119No Hit
TACCCTTACGATGTTCCTGAGTCTGTGGGCTCGAAGATTTCGTTGGGGAC20.10172939979654119No Hit
GATGCGTCCGCGTCTAACATGCACCATTTATTGCATGATACGTGGCATTT20.10172939979654119No Hit
TACCCTTACGATTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAAT20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGGGCAAACATCCCGCCTAA20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAACAGCCCGCCTAATG20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGTGTCTCGAAAATTTCGTTGT20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACAGCCCTCCTAA20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT20.10172939979654119No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGAGATCCCGATCGTGACCTTCGT20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAG20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT20.10172939979654119No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGACATTCCGATCGTGACCTTCGT20.10172939979654119No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGT20.10172939979654119No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)