FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4B03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4B03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA865.168269230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA824.927884615384615No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG794.747596153846153No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT663.9663461538461537No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA462.7644230769230766No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA301.8028846153846152No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA301.8028846153846152No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG271.622596153846154No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA201.201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG171.0216346153846154No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT171.0216346153846154No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT171.0216346153846154No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT160.9615384615384616No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGCTCGAAGATTTCGTTGG150.9014423076923076No Hit
TACCCTTACGATGTTCCTGATTACGCCTGTGGGCTCGAAGATTTCGTTGG130.78125No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG130.78125No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG120.7211538461538461No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT120.7211538461538461No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA110.6610576923076923No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG110.6610576923076923No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG90.5408653846153846No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG90.5408653846153846No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG90.5408653846153846No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC80.4807692307692308No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG80.4807692307692308No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG80.4807692307692308No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA80.4807692307692308No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG70.4206730769230769No Hit
ACACCCAAGCAGATCTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT70.4206730769230769No Hit
ACGCCCAAGCAGATCTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT60.3605769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCCTGGGGCTCGAAGATTTCGTTGGG60.3605769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG60.3605769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA60.3605769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGGGCTCGAAGATTTCGTTGG60.3605769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG60.3605769230769231No Hit
TACCCTTACGATGTTCCTGATTACGTTGTGGGCTCGAAGATTTCGTTGGG50.3004807692307693No Hit
GAGGAAGGCATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA50.3004807692307693No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT50.3004807692307693No Hit
GAGGAGGGAATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA50.3004807692307693No Hit
ACGCCCAAGCAGATTTACAACGATCCGGCCAACCTCTTCGTAGCCAGCTT50.3004807692307693No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTCAGATTTCGTTGGGGA40.2403846153846154No Hit
ACACCCAAGCAGATCTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT40.2403846153846154No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA40.2403846153846154No Hit
ACGCCCAAGCAGATCTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT40.2403846153846154No Hit
GAAGAGGGCATCGTCCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA40.2403846153846154No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA40.2403846153846154No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG40.2403846153846154No Hit
GAGGAGGGAATCGTCCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA40.2403846153846154No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCTCCTAA40.2403846153846154No Hit
ACGCCCAAGCAGATTTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT30.18028846153846154No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG30.18028846153846154No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT30.18028846153846154No Hit
ACACCCAAGCAGATTTACAACGATCCGGCCAACCTCTTCGTAGCCAGCTT30.18028846153846154No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG30.18028846153846154No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTT30.18028846153846154No Hit
ACGCCCAAGCAGATCTACAACGATCCGGCCAACCTCTTCGTAGCCAGCTT30.18028846153846154No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCTTTGTGGACT30.18028846153846154No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT30.18028846153846154No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT30.18028846153846154No Hit
GAAGAGGGAATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA30.18028846153846154No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT30.18028846153846154No Hit
TACCCTTACGATGTTCCTGATTACGCTCGTGGGCTCGAAGATTTCGTTGG30.18028846153846154No Hit
GAAGAGGGCATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA30.18028846153846154No Hit
GAAGAAGGCATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA30.18028846153846154No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT30.18028846153846154No Hit
ACGCCCAAGCAGATCTACAACGATCCCGCCAACCTGTTCGTCGCCAGCTT20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT20.1201923076923077No Hit
GAAGAAGGAATCGTTCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA20.1201923076923077No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCTCCTAA20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGACTCTGT20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGTGTGCTCGAAGATTTCGTT20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCGCTCAGATTTCGTTGGGGA20.1201923076923077No Hit
AATAAGTCAGCATCAAACATGCAACATTTATTGCATGATACGTGGCATTT20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCTTTGGT20.1201923076923077No Hit
GAGGAGGGAATCGTCCCAGGCGGCGGTACTGCTCTTCTGCGCTCTTCCAC20.1201923076923077No Hit
ACGCCCAAGCAGATCTACAACGACCCGGCCAATCTGTTCGTGGCCAGCTT20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGT20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCTAATATTTCTTTGGT20.1201923076923077No Hit
ACACCCAAGCAGATTTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT20.1201923076923077No Hit
GAGGAAGGCATCGTTCCTGGCGGCGGCGTAGCCCTGCTGCGCGCTCGTGC20.1201923076923077No Hit
GAGGAAGGCATCGTCCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGAAGATTTCGTTGGGGACTC20.1201923076923077No Hit
GAGGAGGGAATCGTTCCTGGCGGCGGCGTAGCCCTGCTGCGCGCTCGTGC20.1201923076923077No Hit
GAAGAAGGAATCGTCCCCGGCGGCGGCGTCGCCCTGCTGCGCGCTTCGGA20.1201923076923077No Hit
GAAGAGGGAATCGTTCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGT20.1201923076923077No Hit
GAGGAAGGAATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA20.1201923076923077No Hit
GAGGAGGGCATCGTCCCCGGTGGCGGCACCGCGCTGCTGTACGCGACCAA20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGCTGGGGA20.1201923076923077No Hit
GAGGAGGGCATCGTCCCAGGCGGCGGTACTGCTCTTCTGCGCTCTTCCAC20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA20.1201923076923077No Hit
GAGGAGGGAATCGTCCCCGGCGGCGGTACGGCGTTGCTGTACGCAACCAA20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGTGGACT20.1201923076923077No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGCTCGAAGATTTCGTTGGGG20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG20.1201923076923077No Hit
GAGGAAGGAATCGTCCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA20.1201923076923077No Hit
TACCCTTACGCTGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG20.1201923076923077No Hit
GAGGAAGGCATCGTCCCCGGTGGTGGTACGGCTTTGCTGTACGCGACCAA20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG20.1201923076923077No Hit
TACCCTTACGCTGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTT20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGGAGATTTCGTTG20.1201923076923077No Hit
GAAGAAGGAATCGTCCCAGGCGGCGGTACTGCTCTTCTGCGCTCTTCCAC20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTGGGGACTCTG20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAACTTTCGTTT20.1201923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA20.1201923076923077No Hit
TACCCTTACGCTGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT20.1201923076923077No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)