FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4B01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4B01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2203
Sequences flagged as poor quality0
Sequence length301
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA29313.300045392646393No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA26712.119836586472992No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA1858.397639582387653No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA1597.217430776214254No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA1456.58193372673627No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG592.678166137085792No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA572.587380844303223No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA472.133454380390377No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT261.1802088061734No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG220.9986382206082615No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG200.9078529278256923No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT200.9078529278256923No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA170.7716749886518384No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG170.7716749886518384No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC160.7262823422605538No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT150.6808896958692692No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG140.6354970494779846No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG120.5447117566954154No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCTCCTAA110.49931911030413073No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG100.45392646391284613No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT100.45392646391284613No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG100.45392646391284613No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG90.40853381752156154No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA80.3631411711302769No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCATCCCGCCTAA60.2723558783477077No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCATCCCTCCTAA60.2723558783477077No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGGCTCGAAGATTTCGTTGG60.2723558783477077No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT50.22696323195642307No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCGCCTAA50.22696323195642307No Hit
TACCCTTACGATGTTCCTGATTACGCACAAGAGTTTGCCAGCCCGCCTAA50.22696323195642307No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCGCCTAA50.22696323195642307No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCTCCTAA50.22696323195642307No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA50.22696323195642307No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCATCCCGCCTAA50.22696323195642307No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT50.22696323195642307No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT50.22696323195642307No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA50.22696323195642307No Hit
TACCCTTACGATGTTCCTGATTACGCACAATTCAGTGCCAGCCCGCCTAA50.22696323195642307No Hit
TACCCTTACTATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA50.22696323195642307No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCTCCTAA50.22696323195642307No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA50.22696323195642307No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG50.22696323195642307No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG50.22696323195642307No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCAGCCCGCCTAA40.18157058556513844No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTTCTGCCAGCCCGCCTAA40.18157058556513844No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCTCCTAA40.18157058556513844No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA40.18157058556513844No Hit
TACCCTTACGATGTTCCTGATTACGCATAGAGCAAATGCAGCCCGCCTAA40.18157058556513844No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCTCCTAA40.18157058556513844No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA40.18157058556513844No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG40.18157058556513844No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCCAATGCAGCCCGCCTAA30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAGTGCAGCCCGCCTAA30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGATCTGT30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACAGCCCGCCTAA30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA30.13617793917385385No Hit
TACCCTTACGATGCTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCAGCCCTCCTAA30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCACATGCAGCCCGCCTAA30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGTGTTTGCCAGCCCGCCTAA30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAACAGCCCGCCTAATG30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGGCTCGAAGATTTCGTTG30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTCA30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGAAGATTTCGTTGGGGA30.13617793917385385No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG30.13617793917385385No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)