FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4A09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4A09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1422
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA976.821378340365682No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA886.188466947960619No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA866.047819971870605No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG775.414908579465542No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA725.063291139240507No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT614.289732770745429No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA604.219409282700422No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA493.4458509142053444No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG392.7426160337552745No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG312.180028129395218No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA302.109704641350211No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG241.6877637130801686No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG211.4767932489451476No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG181.2658227848101267No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT161.1251758087201125No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG161.1251758087201125No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT140.9845288326300985No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG120.8438818565400843No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG120.8438818565400843No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT100.7032348804500703No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA100.7032348804500703No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG90.6329113924050633No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC90.6329113924050633No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG90.6329113924050633No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA90.6329113924050633No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGCTCGAAGATTTCGTTGGG90.6329113924050633No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGAAGATTTCGTTGGGGA90.6329113924050633No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG70.49226441631504925No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG70.49226441631504925No Hit
TACCCTTACGATGTTCCTGATTACGCCTGGGGCTCGAAGATTTCGTTGGG60.42194092827004215No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA60.42194092827004215No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG60.42194092827004215No Hit
TACCCTTACGATGTTCCTGATTACGCATTATTTGCTGCCAGCCCGCCTAA50.35161744022503516No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG50.35161744022503516No Hit
TACCCTTACGATGTTCCTGATTTGGGCTCGAAGATTTCGTTGGGGACTCT40.2812939521800281No Hit
TACCCTTACGATGTTCCTGATTACGCACAATTCAGTGCCAGCCCGCCTAA40.2812939521800281No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT30.21097046413502107No Hit
TACCCTTACGATGTTCCTGATTACGCACGATTTGCTGCCAGCCCGCCTAA30.21097046413502107No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA30.21097046413502107No Hit
TACCCTTACGATGTTCCTGATTACGGTGTGGGCTCGAAGATTTCGTTGGG30.21097046413502107No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA30.21097046413502107No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA30.21097046413502107No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG30.21097046413502107No Hit
TACCCTTACGATGTTCCTGATTACGCACGAGCCTGGAACAGCCCGCCTAA30.21097046413502107No Hit
TACCCTTACTATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA30.21097046413502107No Hit
TACCCTTACGATGTTCCTGATTACGTTCGTGGCTCGAAGATTTCGTTGGG30.21097046413502107No Hit
TACCCTTACGATGTTCCTGATTACGCCCTGGGCTCGAATATTTCTTTGGT20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT20.14064697609001406No Hit
TAATTTTAATCGCAGGCATAGTAAGTCCGTTCCTGAGCAAGCGATAATAT20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA20.14064697609001406No Hit
TACCCTTCGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAAT20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCTCCTAA20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGTTGGCTCTAAGATTTCGTT20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT20.14064697609001406No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGGTG20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT20.14064697609001406No Hit
TACTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAATG20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGCTCGAAGATTTCGTTGGGGA20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCTCCTAA20.14064697609001406No Hit
TACCCTTACTATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCCTGTGGGCTCGAAGATTTCGTTGG20.14064697609001406No Hit
TACCCTTACGATGTCCTGATTACACTCGGGCTCGAAGATTTCGTTGGGGA20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGG20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCCTGGCTCGAAGATTTCGTTGGGGA20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTT20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAATATTTCGTTGG20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGT20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCACGATTTTCTGCCAGCCCGCCTAA20.14064697609001406No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTCA20.14064697609001406No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)