FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4A08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4A08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1379
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA1218.774474256707759No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG946.81653372008702No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA946.81653372008702No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT886.381435823060189No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG352.5380710659898478No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG302.1754894851341553No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT231.6678752719361856No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG221.5953589557650472No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG211.5228426395939088No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT201.4503263234227701No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG171.2327773749093547No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG161.160261058738216No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA141.015228426395939No Hit
TACCCTTACGATGTTCCTGATTCGGGGCTCGAAGATTTCGTTGGGGACTC130.9427121102248005No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG120.8701957940536621No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA120.8701957940536621No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG110.7976794778825236No Hit
TACCCTTACGATGTTCCTGATTACGCGTAAATTCCTAGTCGAAGATTTCG110.7976794778825236No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA100.7251631617113851No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAGGCCTGTCAGGCCAAGCTTG100.7251631617113851No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT90.6526468455402465No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT90.6526468455402465No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT90.6526468455402465No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG90.6526468455402465No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC80.580130529369108No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGGGCTCGAAGATTTCGTTGGG80.580130529369108No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA80.580130529369108No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG70.5076142131979695No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA70.5076142131979695No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA70.5076142131979695No Hit
TACCCTTACGATGTTCCTGATTACTGGGCTCGAAGATTTCGTTGGGGACT60.43509789702683105No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA60.43509789702683105No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT60.43509789702683105No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG60.43509789702683105No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCGCCTAA50.36258158085569253No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA50.36258158085569253No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG50.36258158085569253No Hit
TACCCTTACGATGTTCCTGATTACGCCCGGGCTCGAAGATTTCGTTGGGG40.290065264684554No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGATCGAAGATTTCGTTG40.290065264684554No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA40.290065264684554No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG40.290065264684554No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCTCCTAA40.290065264684554No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA40.290065264684554No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG40.290065264684554No Hit
TACCCTTACGATGTTCCTGATTACGGTCTTGGGCTCGAAGATTTCGTTGG40.290065264684554No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA30.21754894851341552No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT30.21754894851341552No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.21754894851341552No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT30.21754894851341552No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA30.21754894851341552No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT30.21754894851341552No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG30.21754894851341552No Hit
TACCCTTACGATGTTCCTGATTACGCTCCTGTTTCTCGAATCTTTCGGTT30.21754894851341552No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC30.21754894851341552No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT30.21754894851341552No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCAGCCCGCCTAA20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGT20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGA20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCGCCTAA20.145032632342277No Hit
TACCCTTACGCTGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGCTTTCGTTG20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGTTCTCGAATCTTTCGTTT20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCGTAAATTCCTAGTCGCAGATTTCG20.145032632342277No Hit
GATGAGTCAGCGTCGAACATGCAACACTTATTGCATGATACGTGGCATTT20.145032632342277No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.145032632342277No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGGTTTCGTTG20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGGGCTCGAAGATTTCGTTGGG20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTGGGGAC20.145032632342277No Hit
TACCCTTACGCTGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTTGTTGGGGACTCTG20.145032632342277No Hit
GATGAGTCTGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTCGGGTCTCGAATCTTTCTTTGGTGACTC20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCATCCCGCCTAA20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGCTCGAAGATTTCGTTGGGGA20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGCTGGGGA20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAGGATTTCGTTGGGGACTCTG20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTG20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG20.145032632342277No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGATTCGTGGTAAAT20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGAC20.145032632342277No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGTTCTCGAATCTTTCGTTG20.145032632342277No Hit
TACCCTTACTATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA20.145032632342277No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)