FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4A04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4A04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1128
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA1079.48581560283688No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA897.890070921985816No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG645.673758865248227No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA534.698581560283688No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT524.609929078014184No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA413.6347517730496453No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA383.368794326241135No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG373.280141843971631No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG353.102836879432624No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA282.4822695035460995No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG262.304964539007092No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT141.2411347517730498No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT121.0638297872340425No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG110.975177304964539No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG100.8865248226950355No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG90.7978723404255319No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG90.7978723404255319No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA70.6205673758865249No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG70.6205673758865249No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA70.6205673758865249No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT60.5319148936170213No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT60.5319148936170213No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA50.44326241134751776No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT50.44326241134751776No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA50.44326241134751776No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA40.3546099290780142No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG40.3546099290780142No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA40.3546099290780142No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA40.3546099290780142No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGAAGATTTCGTTGGGGA40.3546099290780142No Hit
CGGTTCGTGCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACGTTCGT40.3546099290780142No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG30.26595744680851063No Hit
CGGTTCGTCCGCTTCTGCGACGCGTTCGAGATCCCGATCGTGACGTTCGT30.26595744680851063No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA30.26595744680851063No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT30.26595744680851063No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT30.26595744680851063No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA30.26595744680851063No Hit
CGGTTCGTGCGCTTCTGCGATGCGTTCGATATTCCGATCGTGACCTTCGT30.26595744680851063No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG30.26595744680851063No Hit
TACCCTTACGATGTTCCTGATTACGCGTTCGTGGGCTCGAAGATTTCGTT20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCGCCTAA20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTCA20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTATGGCTCGAAGATTTCGTTG20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGACTCTGT20.1773049645390071No Hit
TACCCTTACGTGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGAC20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGA20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGATCTGT20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCGCCTAA20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGACTCGAAGATTTCGTTGGGG20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGATGGGGA20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGACCGCCTAA20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATATTACGCATAGTGCAAATGCAGCCCGCCT20.1773049645390071No Hit
TACCCTTACGATGTTCCTGCTTACGCATAATGTGCAAACAGCCCGCCTAA20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTTCGTTGGGGACTCTGTCTCTTATACACA20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCTAATATTTCTTTGGT20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGCTGGGGA20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCTCCTAA20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCTCGTGGGCTCGAAGATTTCGTTGG20.1773049645390071No Hit
TACCCTTACGATGTTCCTGAATTACGGTTCGTGGCTCGAAGATTTCGTTG20.1773049645390071No Hit
TACCCTTACTATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA20.1773049645390071No Hit
TACCCTTACGCTGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG20.1773049645390071No Hit
TCCCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAATCTTTCGTTT20.1773049645390071No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1773049645390071No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCTCCTAA20.1773049645390071No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)