FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP4A03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP4A03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1797
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT1327.345575959933222No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG864.785754034501948No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT703.8953811908736786No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA653.6171396772398445No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG331.8363939899833055No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG301.669449081803005No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG281.5581524763494714No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG221.2242626599888704No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG221.2242626599888704No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG201.1129660545353366No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT201.1129660545353366No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG191.05731775180857No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT181.001669449081803No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT140.7790762381747357No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG130.7234279354479688No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG130.7234279354479688No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG130.7234279354479688No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT110.6121313299944352No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC100.5564830272676683No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT80.4451864218141347No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGCTCGAAGATTTCGTTGG80.4451864218141347No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA80.4451864218141347No Hit
ACGCCCAAGCAGATCTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT70.38953811908736785No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA70.38953811908736785No Hit
TACCCTTACGATGTTCCTGAGTCTGTGGGCTCGAAGATTTCGTTGGGGAC70.38953811908736785No Hit
ACGCCCAAGCAGATTTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT60.333889816360601No Hit
ACACCCAAGCAGATTTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT60.333889816360601No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT60.333889816360601No Hit
ACACCCAAGCAGATCTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT60.333889816360601No Hit
ACGCCCAAGCAGATCTACAACGATCCCGCCAACCTGTTCGTCGCCAGCTT50.27824151363383415No Hit
GAGGAGGGAATCGTCCCAGGCGGCGGTACTGCTCTTCTGCGCTCTTCCAC50.27824151363383415No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG50.27824151363383415No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT50.27824151363383415No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG50.27824151363383415No Hit
ACGCCCAAGCAGATCTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT50.27824151363383415No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCCGTGGGCTCGAAGATTTCG50.27824151363383415No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCGAAGATTTCGTTGGG50.27824151363383415No Hit
TACCCTTACGATGTTCCTGATTACGCAGTGGGCTCGAAGATTTCGTTGGG50.27824151363383415No Hit
ACGCCCAAGCAGATTTACAACGATCCGGCCAACCTCTTCGTAGCCAGCTT50.27824151363383415No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA50.27824151363383415No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG40.22259321090706735No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGGCTCGAAGATTTCGTTG40.22259321090706735No Hit
ACGCCCAAGCAGATTTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT40.22259321090706735No Hit
GAGGAGGGAATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA40.22259321090706735No Hit
ACACCCAAGCAGATCTACAACGATCCGGCCAACCTCTTCGTAGCCAGCTT40.22259321090706735No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT40.22259321090706735No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA40.22259321090706735No Hit
TACCCTTACGATGTTCCTGATGGGCTCGAAGATTTCGTTGGGGACTCTGT40.22259321090706735No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG40.22259321090706735No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGTGGGCTCGAAGATTTCGTTG30.1669449081803005No Hit
ACACCCAAGCAGATCTACAACGACCCGGCCAACCTGTTCGTGGCCAGCTT30.1669449081803005No Hit
GAAGAGGGAATCGTCCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA30.1669449081803005No Hit
TACCCTTACGATGTTCCTGATTACGCGTTTGGGCTCGAAGATTTCGTTGG30.1669449081803005No Hit
ACGCCCAAGCAGATCTACAACCGCATGCAGAACGTCTATGCCGACCAGCA30.1669449081803005No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG30.1669449081803005No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.1669449081803005No Hit
ACGCCCAAGCAGATCTACAACGATCCGGCCAACCTCTTCGTAGCCAGCTT30.1669449081803005No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG30.1669449081803005No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGGTG30.1669449081803005No Hit
GAGGAAGGAATCGTCCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA30.1669449081803005No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT30.1669449081803005No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCCTGATACGTGTCATTT30.1669449081803005No Hit
GAGGAGGGAATCGTCCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA30.1669449081803005No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG30.1669449081803005No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT30.1669449081803005No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGAC30.1669449081803005No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGAGCTCGAAGATTTCGTTGGG20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGTGCTCGAAGATTTCGTTGGG20.11129660545353368No Hit
ACACCCAAGCAGATTTACACCGATCCGGCCAACCTGTTCGTCGCCAGTTT20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCTGAGCTCTCAGATTTCGTTGGGGA20.11129660545353368No Hit
GAGGAGGGAATCGTCCCCGGTGGCGGCACCGCGCTGCTGTACGCGACCAA20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGACTCTGT20.11129660545353368No Hit
ACACCCAAGCAGATCTACAACGATCCCGCCAACCTGTTCGTCGCCAGTTT20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCGCGGGCTCGAAGATTTCGTTGGGG20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGATCTGT20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTAGTTTGGGACTCTT20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGTCTCGAAGATTTCGTTGGGG20.11129660545353368No Hit
GAGGAAGGAATCGTCCCCGGTGGTGGTACGGCTTTGCTGTACGCGACCAA20.11129660545353368No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGATTCGTGTTAAAT20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTG20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGTGCTCGAAGATTTCGTT20.11129660545353368No Hit
GAAGAGGGAATCGTCCCCGGTGGCGGCACCGCGTTGCTCTATGCCACGAA20.11129660545353368No Hit
GAAGAGGGCATCGTCCCCGGCGGCGGCGTCGCCCTGCTGCGTGCTTCGGA20.11129660545353368No Hit
ACACCCAAGCAGATTTACAACGATCCGGCCAACCTCTTCGTAGCCAGCTT20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCTTTGGT20.11129660545353368No Hit
GAGGAAGGCATCGTCCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA20.11129660545353368No Hit
GAGGAGGGCATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA20.11129660545353368No Hit
GAAGAAGGCATCGTCCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGGTCTTGGGCTCGAAGATTTCGTTGG20.11129660545353368No Hit
ACACCCAAGCAGATCTACAACCGCATGCAGAACGTCTATGCCGACCAGCA20.11129660545353368No Hit
ACACCCAAGCAGATTTACAACCGCATGCAGAACGTCTATGCCGACCAGCA20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGATTCGTGGTAAAT20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTT20.11129660545353368No Hit
GAAGAGGGCATCGTCCCCGGCGGCGGTACGGCTCTGCTGTACGCAACCAA20.11129660545353368No Hit
TACCCTTACGCTGTTCCTGATTACGCTTCGTGTCTCGAATATTTCGTTGT20.11129660545353368No Hit
GAGGAAGGAATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA20.11129660545353368No Hit
TACCCTTACGATGTTCCTGCTTACGCTGTGCTCTCAGATTTCGTTGGGGA20.11129660545353368No Hit
TACCCTTACGCTGTTCCTGCTTACGCTTTGGCTCGAAGATTTCGTTGGGT20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCTTTGGG20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCTTTGGT20.11129660545353368No Hit
GAGGAGGGCATCGTCCCAGGCGGCGGTACTGCTCTTCTGCGCTCTTCCAC20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCGGGCTCGAAGATTTCGTTGG20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAATATTTCGTTGGGGACTCTG20.11129660545353368No Hit
GAAGAGGGCATCGTCCCAGGCGGCGGTACTGCTCTTCTGCGCTCTTCCAC20.11129660545353368No Hit
TACCCTTACGCTGTTCCTGATTACGCTCTACGATTTAGTTTGGGACTCTT20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGGTTTGTGTTCTCGAATCTTTCGTTT20.11129660545353368No Hit
GATGAGTCAGCGTCGCGGCGTACTTTATTTACGGCCAGAAACTCGACCTG20.11129660545353368No Hit
GAGGAGGGCATCGTTCCAGGCGGCGGTACTGCTCTTCTGCGCTCTTCCAC20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAAGATTTCGTTGGT20.11129660545353368No Hit
GAAGAGGGAATCGTCCCCGGTGGTGGTACGGCTTTGCTGTACGCGACCAA20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGCCTCGAATATGTCGTGGT20.11129660545353368No Hit
GAAGAGGGAATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGTCTCGAAGATTGCGTTGT20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGTGGACT20.11129660545353368No Hit
TACCCTTACTATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT20.11129660545353368No Hit
TACCCTTACGCTGTTCCTGCTTACGCTCTACGATTTAGTTTGGGACTCTT20.11129660545353368No Hit
GAAGAGGGCATCGTCCCCGGTGGTGGTACGGCGCTGCTGTACGCGACCAA20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTGGGGACTCTT20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTAAGCTCGAAGATTTCGTTGGGGACTCTG20.11129660545353368No Hit
GATGAGTCAGCGTCGAACAGCTTGAAGCTGCCGACCGCAAGCGCGTCCGC20.11129660545353368No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG20.11129660545353368No Hit
GAGGAAGGCATCGTCCCAGGCGGCGGTACTGCTCTTCTGCGCTCTTCCAC20.11129660545353368No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)