FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT18566.07142857142857No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG248.571428571428571No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT41.4285714285714286No Hit
GAGGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT31.0714285714285714No Hit
GATGAGTCAGCGTCGAACATGCAACATTTAATGCATGATACGTGGCATTT31.0714285714285714No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT31.0714285714285714No Hit
GATGAGTCAGCGTCTAACATGCAACCTTTATTGCATGATACGTGGCATTT20.7142857142857143No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT20.7142857142857143No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTTGCATTT20.7142857142857143No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGCTCAGCTCTAGGACTTCGTT10.35714285714285715No Hit
TACCCTTACGATGTTCCTGATTACGGTTTGATGTCGCTGTATTTGGTTGG10.35714285714285715No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG10.35714285714285715No Hit
GATGAGTCAGCGTCTAACATGCAACATCTATTGCATGAGACGTGGCATTT10.35714285714285715No Hit
GATGAGTCAGCGTAGAACATGCAACATTTATTGCATGATACGTGGCATTT10.35714285714285715No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGTGTTCTCGAATCATTCGTTG10.35714285714285715No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGTTCGAAGCTTGCCTTG10.35714285714285715No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCTCCTAA10.35714285714285715No Hit
GATGAGTCCGCGTCTAACATGCACCCTTTATTGCCTGATACGTGGCATTT10.35714285714285715No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT10.35714285714285715No Hit
GAGGAGTCATCGTCGAACATGCAACATTGATTGCATGATAGGTGGCATTG10.35714285714285715No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGGCGCGACGATTTCGTTGGGG10.35714285714285715No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATCT10.35714285714285715No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATATGTGGCATTT10.35714285714285715No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGCTGG10.35714285714285715No Hit
GATGAGTCAGCGTCGAACCTGCAACATTTATTGCATGTTACGTGGCATTT10.35714285714285715No Hit
GATGAGTCCGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT10.35714285714285715No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT10.35714285714285715No Hit
TACCCTTACGATGTTCCTGATTACGCCTGGAAGATTCCTTGGGGGATCTG10.35714285714285715No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.35714285714285715No Hit
GATGAGTCAGCGTCGAACATGCATCATTTATTGCATGATACGTGGCATTT10.35714285714285715No Hit
CTGTAACGATAAATGGGTCATCTAAAGAGTCTGGAGCTGGGATTGACTCG10.35714285714285715No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT10.35714285714285715No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT10.35714285714285715No Hit
GAGGAGTCAGCGTCGAACATGCACCATTTATCTCATGATACGTGGCATTT10.35714285714285715No Hit
TACCCTTACGATGTTCCTGATTACGCTCGCCGCGATTGAATCTTTCGTTT10.35714285714285715No Hit
TACCCTTACGATGTTCCTGATTACGCACAATTAAGTCGCTGCGCGCCTAA10.35714285714285715No Hit
GAAGCGTCCGCGTCTAACATGAACCCTTTATCTCATGATACGTGTCATTT10.35714285714285715No Hit
GATGAGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT10.35714285714285715No Hit
TACCCTTACGATGTTCCTGATTACGCCGGGAGCTCGCAGATTTCGTTGGG10.35714285714285715No Hit
GATGAGTCAGCATCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.35714285714285715No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGAGGCATTT10.35714285714285715No Hit
GATGAGCCAGCGTCGAACATGCAACATTTAATGCATGATACGTGGTATTT10.35714285714285715No Hit
GAGGAGACAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.35714285714285715No Hit
TACCCTTACGATGTTCCTGATTACGCCGCGACGCGTACATTTCGGAGTCT10.35714285714285715No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.35714285714285715No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGATTACTCTG10.35714285714285715No Hit
GATGAGTCAGAGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.35714285714285715No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTTGCATTT10.35714285714285715No Hit
CTGTAAAAATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.35714285714285715No Hit
TACCCTTACGATGTTCCTGATTACACTGAGATCGAAGATTTCGCGGCGGA10.35714285714285715No Hit
GATGAGTCAGCGTCAAATATGCAACATTTATTTCATGATACGTGGCATTT10.35714285714285715No Hit
TACCCTTACGATGTTCCCGATTACGCCTCGGTCCCTCGAAGGTCTCGCTG10.35714285714285715No Hit
GATGAGTCAGAGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT10.35714285714285715No Hit
GATGAGTCCGCGTCGAACATGAACCCTTTATTTCATGATACGTGTCATTT10.35714285714285715No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA10.35714285714285715No Hit
GATGCGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT10.35714285714285715No Hit
GATGAGTCAGCGTCGAACATGCAAAATTTATTGCATGATACGTGGCATTT10.35714285714285715No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATAATACGTGGCATTT10.35714285714285715No Hit
GATGAGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT10.35714285714285715No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.35714285714285715No Hit
GAGGCGTCCGCGTCTAACATGAACCCTTTATGACATGATACGTGACAGTT10.35714285714285715No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)