FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3H04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3H04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1139
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20117.647058823529413No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA1028.955223880597014No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG393.4240561896400354No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT302.633889376646181No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG262.28270412642669No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG262.28270412642669No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT151.3169446883230904No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA141.2291483757682178No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG131.141352063213345No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG110.9657594381035997No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG110.9657594381035997No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG90.7901668129938543No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC90.7901668129938543No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA90.7901668129938543No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG90.7901668129938543No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA80.7023705004389815No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG60.5267778753292361No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG60.5267778753292361No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG60.5267778753292361No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGTGGGCTCGAAGATTTCGTTG50.4389815627743635No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG50.4389815627743635No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT50.4389815627743635No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG50.4389815627743635No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCGAAGATTTCGTTGGG50.4389815627743635No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGCTCGAAGATTTCGTTGG40.35118525021949076No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG40.35118525021949076No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT40.35118525021949076No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT30.2633889376646181No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT30.2633889376646181No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG30.2633889376646181No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA30.2633889376646181No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT30.2633889376646181No Hit
GATGAGTCAGCGTCGAACATGCAACATTTAATGCATGATACGTGGCATTT30.2633889376646181No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG30.2633889376646181No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA30.2633889376646181No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGGTG30.2633889376646181No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG30.2633889376646181No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT30.2633889376646181No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGCTGGGGA30.2633889376646181No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA30.2633889376646181No Hit
TACCCTTACGATGTTCCTGATTAGTGGGCTCGAAGATTTCGTTGGGGACT30.2633889376646181No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCTAATATTTCTTTGGT30.2633889376646181No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG30.2633889376646181No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG30.2633889376646181No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGTGCTCTAAGATTTCGTT30.2633889376646181No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACATGGCATTT30.2633889376646181No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT30.2633889376646181No Hit
TACCCTTACGATGTTCCTGATTACGCTTCCTGTTCTCGAATCTGTCGTTT20.17559262510974538No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTTGCATTT20.17559262510974538No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.17559262510974538No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.17559262510974538No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT20.17559262510974538No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCGCTCAGATTTCGTTGGGGA20.17559262510974538No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTAGTTTGGGACTCTT20.17559262510974538No Hit
TACCCTTACGCTGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA20.17559262510974538No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGATCTAAGATTTCGTTGGGGA20.17559262510974538No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.17559262510974538No Hit
GATGCGTCCGCGTCTAACATGAACCCTTTATTTCCTGATACGTGTCATTT20.17559262510974538No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT20.17559262510974538No Hit
GATGAGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT20.17559262510974538No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGCCATTT20.17559262510974538No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT20.17559262510974538No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT20.17559262510974538No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT20.17559262510974538No Hit
GATGAGTCAGCGTAGAACATGCAACATTTATTGCATGATACGTGGCATTT20.17559262510974538No Hit
TACCCTTACGCTGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG20.17559262510974538No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC20.17559262510974538No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGGCTCGAAGATTTCGTTG20.17559262510974538No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTC20.17559262510974538No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.17559262510974538No Hit
TACCCTTACGCTGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT20.17559262510974538No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT20.17559262510974538No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)