FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3G10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3G10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47
Sequences flagged as poor quality0
Sequence length301
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG714.893617021276595No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT48.51063829787234No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA36.382978723404255No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG36.382978723404255No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG24.25531914893617No Hit
TACCCTTACGATGTTCCTGATAACGCTGAACCCTTCTGGCTCTTATTCAG12.127659574468085No Hit
TACCCTTACGATGTTCCTGATGATGCCAAAGCTCGACCCTTCCGTTTCTT12.127659574468085No Hit
GATGCGTCCGCGTCTAACATGTACCCTCTATTTCCTGATACGTGTCATTT12.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGCTTTCGTTG12.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTAGTTTGGGACTCTA12.127659574468085No Hit
TACCCTTACGCTGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT12.127659574468085No Hit
TACCCTTACGATGTTCCTGACAACGCTGAAACCTTCTGTCTCTTATACAC12.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT12.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGCAGATTTCGTTGGGACTCTG12.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGG12.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCTCTTAGTCCGCGGCAGTCGTTGAC12.127659574468085No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTAGCATTT12.127659574468085No Hit
GATGAGTCATCGTCAAACATGCAACATTTATTGCATGATACGTGGCATTT12.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGATCTGC12.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG12.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCTCAACGTGTTAGTTTAGGAATCTG12.127659574468085No Hit
TACCCTTACGATGTTCCTGTTACGCCGTTGGCTCGAAGATTTCGTTGGGT12.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA12.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGAGTTAGTTTGGGACTCTT12.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT12.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA12.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGACTTCGTCAGAGATTGTG12.127659574468085No Hit
GATGAGTCAGCGTCGAACAAGCAACATTTATTGCATGATACGTGGCATTT12.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTAGTTGGGGA12.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGGCTCTAATAGTTCGCTGGG12.127659574468085No Hit
CCGAACATATACATGTTTCATCTAAAGAGTATGGAGCTGGGATTGACTCG12.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT12.127659574468085No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACATGGCATTT12.127659574468085No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)