FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3G05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3G05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1939
Sequences flagged as poor quality0
Sequence length301
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT26213.512119649303763No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA22211.44920061887571No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA22011.346054667354306No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA1377.065497679216091No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA1055.415162454873646No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA864.43527591542032No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA572.939659618359979No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA572.939659618359979No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG512.630221763795771No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA140.7220216606498195No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG130.6704486848891181No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCATCCCGCCTAA100.5157297576070139No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA90.46415678184631254No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG90.46415678184631254No Hit
TACCCTTACGATGTTCCTGATTACGTTCGGGGCTCGAAGATTTCGTTGGG70.36101083032490977No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG70.36101083032490977No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT70.36101083032490977No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT70.36101083032490977No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG70.36101083032490977No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG60.30943785456420836No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA60.30943785456420836No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG60.30943785456420836No Hit
TACCCTTACGATGTTCCTGATTACGCACAATTCAGTGCCAGCCCGCCTAA60.30943785456420836No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG60.30943785456420836No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG60.30943785456420836No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG50.25786487880350695No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG50.25786487880350695No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA50.25786487880350695No Hit
TACCCTTACGATGTTCCTGATGGGCTCGAAGATTTCGTTGGGGACTCTGT50.25786487880350695No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT50.25786487880350695No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCAGCCCGCCTAA40.2062919030428056No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTCAGATTTCGTTGGGGA40.2062919030428056No Hit
TACCCTTACGATGTTCCTGATTACTGGGCTCGAAGATTTCGTTGGGGACT40.2062919030428056No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACAGCCCGCCTAA40.2062919030428056No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCATCCCGCCTAA40.2062919030428056No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG40.2062919030428056No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA40.2062919030428056No Hit
TACCCTTACGATGTTCCTGATTACGCACAAGAGTTTGCCAGCCCGCCTAA30.15471892728210418No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCGCTCAGATTTCGTTGGGGA30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCATCCCTCCTAA30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGCATAGAGCAAATGCAGCCCGCCTAA30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGCATTAGCCTGTAACATCCCTCCTAA30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCTCCTAA30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCTCATAA30.15471892728210418No Hit
GCTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCTCCTAA30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA30.15471892728210418No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTCA30.15471892728210418No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG30.15471892728210418No Hit
TACCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAAT20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA20.1031459515214028No Hit
TACCCTTACGCTGTTCCTGATTACGCTGGGCTCTCAGATTTCGTTGGGGA20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCTCCTAA20.1031459515214028No Hit
TTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAATGAGC20.1031459515214028No Hit
TACCCTTACGATGTTCCTGCTTACGCATAGTGCAAATGCATCCCTCCTAA20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCGCCTAA20.1031459515214028No Hit
GATAAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGAGTCTTGGGCTCGAAGATTTCGTTG20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTCCGCATAATTCAGTGCCAGCCCGCCTAA20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGCATTAGCCTGTAACAGCCCGCCTAA20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGCTCGAAGATTTCGTTGGGG20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGCTTGACGATTTCGTTTGGGACTCTT20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTG20.1031459515214028No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGAATGATACGTGGCATTT20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCAGCCCTCCTAA20.1031459515214028No Hit
GATGAGTCAGCGTCGAACATGCAACATTTACTGCATGATACGTGGCATTT20.1031459515214028No Hit
TACCCTTACTATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGCACAATTCAGTGCCATCCCTCCTAA20.1031459515214028No Hit
GATGAGTCAGCGTCGAACATGAAACATTTATTGCATGATACGTGGCATTT20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGCATTAGCCTGTAACAGCCCTCCTAA20.1031459515214028No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCTCCTAA20.1031459515214028No Hit
TACCCTTACTATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA20.1031459515214028No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTTGGGCTCGAAGCTTTCGTTT20.1031459515214028No Hit
GATGAGTCAGCGTCGAACGTGCAACATTTATTGCATGATACGTGGCATTT20.1031459515214028No Hit
TACCCTTACGCTGTTCCTGATTACGCATAAGATTTTGCCATCCCTCCTAA20.1031459515214028No Hit
TACCCTTACGCTGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA20.1031459515214028No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA20.1031459515214028No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)