FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3G04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3G04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1074
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT18517.225325884543764No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA918.472998137802607No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG706.517690875232775No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG353.2588454376163876No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG181.675977653631285No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG161.48975791433892No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG161.48975791433892No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG151.3966480446927374No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG141.303538175046555No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT131.2104283054003724No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG111.0242085661080074No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG111.0242085661080074No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT111.0242085661080074No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA111.0242085661080074No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT70.6517690875232774No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT70.6517690875232774No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT70.6517690875232774No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG70.6517690875232774No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG60.5586592178770949No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGCTCGAAGATTTCGTTGG50.4655493482309125No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG50.4655493482309125No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT50.4655493482309125No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA40.37243947858473No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC40.37243947858473No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTT40.37243947858473No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTCAGATTTCGTTGGGGA30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCAGTGGGCTCGAAGATTTCGTTGGG30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA30.27932960893854747No Hit
GATGAGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT30.27932960893854747No Hit
AATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGAAGATTTCGTTGGGGA30.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCTGCTCGAAGATTTCTTTGTGGATT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCTGGTCTCGAATCTTTCGTTT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA20.186219739292365No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.186219739292365No Hit
TACCCTTACGCTGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTT20.186219739292365No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCTGTTCTCGAATCTTTCGTTT20.186219739292365No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTCGCGTGGGCTCGAAGATTTCGTTGGGGA20.186219739292365No Hit
GATGAGTCAGCGTCGAACATGCAACATTTAATGCATGATACGTGGCATTT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTGGCTCGAAGATTTCGTTGGGGAC20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGG20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGTCTCGAATATTGCGTTGT20.186219739292365No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATGTCCTGATACGAGTCATTT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGGTTTCGTTGG20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGTGGACTCTG20.186219739292365No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGGTCTTGGGCTCGAAGATTTCGTTGG20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA20.186219739292365No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACATGGCATTT20.186219739292365No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTG20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGGCTCGAAGATTTCGTTG20.186219739292365No Hit
TACCCTTACGATGTTCCTGATTACGCGGTCTCGAAGATTTCGTTGGGGAC20.186219739292365No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGAATTT20.186219739292365No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)