FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3G03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3G03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length301
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1325.0No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG35.769230769230769No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT23.8461538461538463No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCCAAGATTTCGTTGGGT11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCGTGTGGTTGTAGAATCAGTCGGTT11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGAGTTAGTTTGGGACTCTA11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCAGTGGGCTCGAAGATTTCGTTGGG11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGAGTTCGTTTGGGACTCTA11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTG11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCCGGGTGTCCTAGAATTACTTTGGG11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGGAGGTTTCGGTGGGG11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCGCTACGATTGCGGTTGGGGCTCTG11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTAGTTTGGGACTCTT11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCCTTTGGTCTCGTAGATTTCGTTGT11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGTTGAAGATGGGGACGGGG11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGAGTTCGTTTGGGACTCTG11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATGACACAGTTGAACCGTTGTTTTTGTTATA11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTTTCGCGGATCTCGCGTTG11.9230769230769231No Hit
GATGAGTCAGCGTCGAACATGCAATATTTATTGCATGATACGTGGCATTT11.9230769230769231No Hit
GATGAGTCAGCGTCGAACATGCAAAATTTATTACATGATACGTGGCATTT11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGATCGCAGATTTCGTTGGGGA11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGTTTTGGTGGGGG11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTCCACGTTGTCTCTTCGATGTGTA11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCGCGAAGATTTCGGTGGGG11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCGCGTAGATTTCGTTGGGGACTCTT11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTTTGCTCTAAGATTTCGTT11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCCGGGGGCTCTAAGATTTCGTTGGG11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTGAGCTCGAAGATTTCGTTGGGGA11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGCTCTGG11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTCTTGGTGGTAAAGGTAGGTG11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGCTCGATTCTTTGGTCTTTGT11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGGTGCGCTCGGAGATTTCGTTGGGGA11.9230769230769231No Hit
CTGTAAAGATAAAAGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG11.9230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCGCGACGTTTTCGTTTGGGTCTCTG11.9230769230769231No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)