FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3G02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3G02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1743
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30317.38382099827883No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA1609.179575444635686No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA1146.540447504302927No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG915.220883534136546No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT704.016064257028113No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA412.352266207687894No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG402.2948938611589216No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA291.663798049340218No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA281.6064257028112447No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT241.376936316695353No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG211.2048192771084338No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA211.2048192771084338No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG201.1474469305794608No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG181.0327022375215147No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA170.9753298909925416No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG150.8605851979345954No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG140.8032128514056224No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA130.7458405048766494No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG120.6884681583476765No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGATCGAAGATTTCGTTG110.6310958118187034No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC110.6310958118187034No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG110.6310958118187034No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT100.5737234652897304No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT90.5163511187607573No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT90.5163511187607573No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT90.5163511187607573No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG90.5163511187607573No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG90.5163511187607573No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG80.45897877223178424No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA70.4016064257028112No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG70.4016064257028112No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA50.2868617326448652No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA50.2868617326448652No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACAGCCCGCCTAA50.2868617326448652No Hit
TACCCTTACGATGTTCCTGATTACGCTGGTCTCGAAGATTTCGTTGGGGA50.2868617326448652No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT50.2868617326448652No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGCTTTCGTTG40.22948938611589212No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.22948938611589212No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG40.22948938611589212No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG40.22948938611589212No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG30.17211703958691912No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG30.17211703958691912No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCGTTGGCTCGAAGATTTCGTT30.17211703958691912No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA30.17211703958691912No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG30.17211703958691912No Hit
GATGAGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT30.17211703958691912No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT30.17211703958691912No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG30.17211703958691912No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA30.17211703958691912No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.17211703958691912No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCATCCGATTCGTGTTAAAT30.17211703958691912No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT30.17211703958691912No Hit
TACCCTTACGATGTTCCTGATTACGCCCGAAGATTTCGTTGGGGACTCTG30.17211703958691912No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGCCGAGTCGTGTTAAAT20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGTGGGCTCGAAGATTTCGTTG20.11474469305794606No Hit
TACCCTTACGATGTTCCTGCTTACGCCTCGAAGATTTCTTTGGGGCCTCT20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGGTTTCGTTGGGGACTCTG20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGATCTGT20.11474469305794606No Hit
GATGAGTCAGCGTCGAACATGCAACATATATTGCATGATACGTGGCATTT20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAACT20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCAGTGGGCTCGAAGATTTCGTTGGG20.11474469305794606No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGTCATTT20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGACAGACGAGTCGTGGTAAAT20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCTCCTAA20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGTGCTCGAAGCTTTCGTTG20.11474469305794606No Hit
GATGAGCCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGGTT20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGCTGG20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGGTG20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGAGGCTCGAAGATTTCGTTGG20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGTGCTCGAAGATTTCGTT20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA20.11474469305794606No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT20.11474469305794606No Hit
GATGAGTCAGCGTCGAATATGCAACATTTATTGCATGATACGTGGCATTT20.11474469305794606No Hit
GATGAGTCAGAGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.11474469305794606No Hit
GCTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCACAATTCAGTGCCAGCCCGCCTAA20.11474469305794606No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGAGGCATTT20.11474469305794606No Hit
TACCCTTACTATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGAAGATTTCGTTGGGGACTC20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAATATTTCGTTGGG20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTG20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGGAGATTTCGTTG20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAACAGCCCGCCTAATG20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTTGTTG20.11474469305794606No Hit
GATGCGTCCGCGTCTAACATGCACCCTCTATTGCATGATACGTGTCATTT20.11474469305794606No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTT20.11474469305794606No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGAC20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCTCCTAA20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGT20.11474469305794606No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGGCATTT20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGTCTCGAATATTTCGTTGT20.11474469305794606No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTTGTTCTCGAATCTTTCGTTG20.11474469305794606No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)