FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3G01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3G01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1348
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT28621.216617210682493No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA795.86053412462908No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA755.563798219584569No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA685.044510385756676No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG533.931750741839762No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA493.635014836795252No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA423.115727002967359No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA392.893175074183976No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA352.596439169139466No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG221.6320474777448073No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG171.261127596439169No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG110.8160237388724036No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT110.8160237388724036No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT110.8160237388724036No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG110.8160237388724036No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG90.6676557863501483No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA80.5934718100890208No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT80.5934718100890208No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG60.44510385756676557No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA60.44510385756676557No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT60.44510385756676557No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA60.44510385756676557No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG60.44510385756676557No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT50.370919881305638No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG50.370919881305638No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG50.370919881305638No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT50.370919881305638No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT40.2967359050445104No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCCTGATACGTGTCATTT40.2967359050445104No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG40.2967359050445104No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCATTT30.22255192878338279No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA30.22255192878338279No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT30.22255192878338279No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT30.22255192878338279No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG30.22255192878338279No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA30.22255192878338279No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG30.22255192878338279No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA30.22255192878338279No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT30.22255192878338279No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGGGGCTCGAAGATTTCGTTGG30.22255192878338279No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG30.22255192878338279No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG30.22255192878338279No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCGCCTAA20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCGCCTAA20.1483679525222552No Hit
GAGGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGAGCTCGAAGATTTCGTTGGG20.1483679525222552No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTTGCATTT20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCGCCTAA20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCATTATTTGCTGCCAGCCCGCCTAA20.1483679525222552No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCCTGATACGTGGCATTT20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACAGCCCGCCTAA20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTTCTGCCAGCCCGCCTAA20.1483679525222552No Hit
GATGAGTCAGCGTCGAACATGCAACATTTAATGCATGATACGTGGCATTT20.1483679525222552No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGTCATTT20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA20.1483679525222552No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT20.1483679525222552No Hit
GATGCGTCCGCGTCTAACATGAACCCTTTATTTCCTGATACGTGTCATTT20.1483679525222552No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATAATACATAGCATTT20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG20.1483679525222552No Hit
GCTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACATCCCGCCTAA20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCTCGTGGGCTCGAAGATTTCGTTGG20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCATAAATCAGTGCCAGCCCGCCTAA20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGTGCTCGAAGATTTCGTTG20.1483679525222552No Hit
GATGAGTCAGCGTCGAACAAGCAACATTTATTGCATGATACGTGGCATTT20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCTCCTAA20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGGTCGAAGATTTCGTTGGGGA20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAACAGCCCGCCTAATG20.1483679525222552No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGTCATTT20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTAGTCTCTGGGCTCGAAGATTTCGTTGGG20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG20.1483679525222552No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCGTTGGT20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGAC20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTCA20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCATCCCTCCTAA20.1483679525222552No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGGCTCGAAGATTTCGTTGGGT20.1483679525222552No Hit
TACCCTTACGCTGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA20.1483679525222552No Hit
TACCCTTACGGTGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA20.1483679525222552No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)