FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3F12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3F12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18
Sequences flagged as poor quality0
Sequence length301
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT633.33333333333333No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG211.11111111111111No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCATTT15.555555555555555No Hit
TACCCTTACGATGTTCCTGATTACGCATGATGTGCAAACAGCCCTCCTAA15.555555555555555No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCGTGATACGTGGCATTT15.555555555555555No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT15.555555555555555No Hit
TACCCTTACGATGTTCCTGATTACGCATGACGTGAAAACATCCCTCCTAA15.555555555555555No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG15.555555555555555No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGAGGCATTT15.555555555555555No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTAGGGCTCGAAGATTTCGTTG15.555555555555555No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA15.555555555555555No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT15.555555555555555No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)