FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3E10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3E10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1036
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA10710.328185328185329No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA938.976833976833976No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA908.687258687258687No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA696.660231660231661No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA595.6949806949806945No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG585.598455598455598No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA504.826254826254826No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA333.1853281853281854No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT222.1235521235521233No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG121.1583011583011582No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG111.0617760617760617No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG100.9652509652509652No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG90.8687258687258688No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG60.5791505791505791No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG50.4826254826254826No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTCA40.3861003861003861No Hit
TACCCTTACGATGTTCCTGCTTACGCATGAGCCTGGAACAGCCCGCCTAA40.3861003861003861No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG40.3861003861003861No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA40.3861003861003861No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG40.3861003861003861No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA40.3861003861003861No Hit
TACCCTTACGCTGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA40.3861003861003861No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCATCCCGCCTAA30.28957528957528955No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG30.28957528957528955No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCTGGGCTCGAAGATTTCGTTG30.28957528957528955No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG30.28957528957528955No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA30.28957528957528955No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG30.28957528957528955No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA30.28957528957528955No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG30.28957528957528955No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCTCCTAA30.28957528957528955No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGGCTCGAAGATTTCGTTG30.28957528957528955No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGGTTGCCAGCCCGCCTAA30.28957528957528955No Hit
TACCCTTACGCTGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA30.28957528957528955No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA30.28957528957528955No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCAGCCCGCCTAA20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCTCCTAA20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA20.19305019305019305No Hit
TACCCTTACGATGTTCCTGCTTACGCATGATTTGCTGCCAGCCCGCCTAA20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCTCCTAA20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA20.19305019305019305No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACATCCCGCCTAA20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGCTGGGGA20.19305019305019305No Hit
CTGTAAAGATAAATGGGGCATCTAAAGAGTATGGAGCTGGGATTGACTCG20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCACAATTCAGTGCCAGCCCGCCTAA20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGTGTTTGCCAGCCCGCCTAA20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCATAAATCAGTGCCAGCCCGCCTAA20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCACTATTTGCTGCCATCCCGCCTAA20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGCACAGCCCGCCTAA20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCGCCTAA20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAATGCAGCCCGCCT20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCATTATTTGCTGCCAGCCCGCCTAA20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCCTTG20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTTCTGCCAGCCCGCCTAA20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT20.19305019305019305No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCTCCTAA20.19305019305019305No Hit
TACCCTTACGCTGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA20.19305019305019305No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)