FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3E01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3E01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12
Sequences flagged as poor quality0
Sequence length301
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGGCGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGTCATTT18.333333333333332No Hit
TACCCTTACGATGTTCCTGATTACGCGGGGTCGAAGATTTCTTTGGTGGC18.333333333333332No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGCGCGAGTATTTCGTTT18.333333333333332No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG18.333333333333332No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGAGTGACTCG18.333333333333332No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTCGAATCTTCGATGTGTA18.333333333333332No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG18.333333333333332No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCAAAGTTTTGGTTGGGA18.333333333333332No Hit
CTGTAAAGATAAATGGGTCATCTAAATCGTATGGAGCTGGTATTGCTTCG18.333333333333332No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT18.333333333333332No Hit
TATTATTAATCTGTGCCTCATCTACATCTTATGCATAGATTTTGGCCTGT18.333333333333332No Hit
TACCCTTACGCTGTTCCTGATTACGCTTTCGTTTGCTCTAAGATTTAGTT18.333333333333332No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)