FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3D11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3D11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1464
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT29019.808743169398905No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA1449.836065573770492No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA724.918032786885246No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA624.2349726775956285No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA624.2349726775956285No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA594.030054644808743No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA553.7568306010928962No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG533.6202185792349724No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG211.4344262295081966No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG211.4344262295081966No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG181.2295081967213115No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG130.8879781420765027No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG130.8879781420765027No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG110.7513661202185792No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA110.7513661202185792No Hit
TACCCTTACGATGTTCCTGATTTCGTTGGGGACTCTGCTCTTATTCGAAG100.6830601092896175No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG100.6830601092896175No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA70.47814207650273227No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGGGCTCGAAGATTTCGTTGG70.47814207650273227No Hit
TACCCTTACGATGTTCCTGATTACGTTCGGGCTCGAAGATTTCGTTGGGG70.47814207650273227No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG70.47814207650273227No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGAAGATTTCGTTGGGGA70.47814207650273227No Hit
TACCCTTACGATGTTCCTGATTACGCAGTGGGCTCGAAGATTTCGTTGGG60.4098360655737705No Hit
TACCCTTACGATGTTCCTGATTACGTTGGGGACTCTGCTCTTATTCGAAG60.4098360655737705No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT60.4098360655737705No Hit
TACCCTTACGATGTTCCTGATTACGCACGATTTGCTGCCAGCCCGCCTAA50.34153005464480873No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT50.34153005464480873No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA50.34153005464480873No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.273224043715847No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTTCTGCCAGCCCGCCTAA40.273224043715847No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT40.273224043715847No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG30.20491803278688525No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT30.20491803278688525No Hit
TACCCTTACGATGTTCCTGATTACGAAGATTTCGTTGGGGACTCTGTCTC30.20491803278688525No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA30.20491803278688525No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT30.20491803278688525No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG30.20491803278688525No Hit
TACCCTTACGATGTTCCTGATGGGCTCGAAGATTTCGTTGGGGACTCTGT30.20491803278688525No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG30.20491803278688525No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTT30.20491803278688525No Hit
TACCCTTACGATGTTCCTGATTAGTGGGCTCGAAGATTTCGTTGGGGACT30.20491803278688525No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT30.20491803278688525No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG30.20491803278688525No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.20491803278688525No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCGAAGATTTCGTTGGG30.20491803278688525No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTCAGATTTCGTTGGGGA20.1366120218579235No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTATGGCTCGAAGATTTCGTTG20.1366120218579235No Hit
TACCCTTACGATTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAAT20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGGGCAAACAGCCCGCCTAA20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG20.1366120218579235No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA20.1366120218579235No Hit
GATGAGTCAGCGTCGAACATGCAACATTTAATGCATGATACGTGGCATTT20.1366120218579235No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGTCATTT20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGCTTTCGTTG20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGTCTCGAATATTTCGTTGT20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGGGCTCGAAGATTTCGTTGGG20.1366120218579235No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCCTGATACGTGTCATTT20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTTCGTTGGGGACTCTGTCTCTTATACACA20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCATCCCGCCTAA20.1366120218579235No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCCTGATACGTGTCATTT20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCACAATTCAGTGCCAGCCCGCCTAA20.1366120218579235No Hit
TACCCTTACTATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGGCTCGAAGATTTCGTTGG20.1366120218579235No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA20.1366120218579235No Hit
GATGAGTCAGCGTCGAACATGAAACATTTATTGCATGATACGTGGCATTT20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCACGAGCCTGGAACAGCCCGCCTAA20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGAC20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTCA20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGAAGATTTCGTTGTGGACTCTGTCTC20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG20.1366120218579235No Hit
TACCCTTACGATGTTCCTGATTTCGTTGTGGACTCTGCTCTTATTCGAAG20.1366120218579235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)