FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3D09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3D09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT3029.126213592233007No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG54.854368932038835No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG43.8834951456310676No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG32.912621359223301No Hit
GCTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT32.912621359223301No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT32.912621359223301No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT21.9417475728155338No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT21.9417475728155338No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA21.9417475728155338No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT21.9417475728155338No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCATTGGT10.9708737864077669No Hit
GATGAGTCAGCGTAGAACATGCAACATTTATTGCATGATACGTGGCATTT10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCGTTCGTGGGCTCGAAGATTTCGTT10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGACTCGAATATCGCGTAGA10.9708737864077669No Hit
GAAGCGTCCGCGTCTAACATGAACCCTTTATTACCTGATACGAGTCGATT10.9708737864077669No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATGGCATGATACGGGTCATTT10.9708737864077669No Hit
TACCCTTACGATGTTCCTGAGTACCCTTACGATGTTCCTGATTACGCGTG10.9708737864077669No Hit
GATGCGTCAGCGTCGAACATGCAACATTATTGCATGATACGTGGCATTTC10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGATGATTTCGTTG10.9708737864077669No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTTCATGATACGTGGCATTT10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCACATGCATCCCTCCTAA10.9708737864077669No Hit
GATGCGTCCGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT10.9708737864077669No Hit
GATGAGTCAGCGCCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.9708737864077669No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG10.9708737864077669No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGTTACGTGGCATTT10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCCTTG10.9708737864077669No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCAAGATACGTGGCATTT10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC10.9708737864077669No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT10.9708737864077669No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCATTT10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCGTCATGGGCACGAAGTTTTCGTTG10.9708737864077669No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGACACGTGGCATTT10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCCCCGTGTTCTCGAATCTTTCGTTT10.9708737864077669No Hit
CTGTAAAGATCAATGGGACATCTAAAGAGTATGGATCTGGGATTGACTCG10.9708737864077669No Hit
TACCCTTACGCTGTTCCTGCTTACGCTCCGTGGTCTCGAATCTTTCGTTT10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCGCTACGAGTTCGTTGGGGACTCTG10.9708737864077669No Hit
TACCCTTACGATGTTCCTGCTTACGCCTCGTGGGCTCGAAGCTTTCGTTG10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT10.9708737864077669No Hit
GATGAGTCCGAGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCTTTGGG10.9708737864077669No Hit
GATGAGTCAGCGTCGAGCATGCAACATTTATTGCATGATACGTGGCATTT10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCTCCTAA10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCTTTGTGGACT10.9708737864077669No Hit
GATGAGTAAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT10.9708737864077669No Hit
GATGAGTCAGCGTCGAACATGCAACATTGATTGCATGATACGTGGCATTG10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCGGAGTTCTCGGGGGG10.9708737864077669No Hit
TACCCTTACGCTGCTCCCGTGTACGCTTTATGACTTGGTTTGGGACGCTT10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGACTAATTCGTTTGGGAG10.9708737864077669No Hit
CAGGCCATCGATATTTTTCATCTCACGCTTAGTGCTATTGTATATCCTCT10.9708737864077669No Hit
GATGAGTCAGCGTCGAACATGCAACATTGATTGCATGATACGTGGCATTT10.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC10.9708737864077669No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT10.9708737864077669No Hit
GATGCGTCCGCGTCTAACATGAACCCTCTATTGCCTGATAGATGTCATTG10.9708737864077669No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTTCATGATACGTGGCATTT10.9708737864077669No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)