FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3D06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3D06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1790
Sequences flagged as poor quality0
Sequence length301
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA48527.09497206703911No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT25914.469273743016759No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA1277.094972067039105No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA995.53072625698324No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA915.083798882681564No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA522.905027932960894No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA442.4581005586592175No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG241.3407821229050279No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG221.2290502793296088No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA201.1173184357541899No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA160.8938547486033519No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT150.8379888268156425No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCGCCTAA140.782122905027933No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG120.6703910614525139No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG110.6145251396648044No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG90.5027932960893855No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT80.44692737430167595No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA80.44692737430167595No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG70.3910614525139665No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT70.3910614525139665No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA70.3910614525139665No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG70.3910614525139665No Hit
TACCCTTACTATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA60.33519553072625696No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG60.33519553072625696No Hit
TACCCTTACGATGTTCCTGATTACGCACAAGAGTTTGCCAGCCCGCCTAA50.27932960893854747No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT50.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCGCCTAA50.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG50.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG50.27932960893854747No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCATCCCGCCTAA40.22346368715083798No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG40.22346368715083798No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCTCCTAA40.22346368715083798No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG40.22346368715083798No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.22346368715083798No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG40.22346368715083798No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCCAATGCAGCCCGCCTAA30.16759776536312848No Hit
TACCCTTACGATGTTCCTGCTTACGCATAGTGCAAATGCAGCCCGCCTAA30.16759776536312848No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGCTCGAAGATTTCGTTGG30.16759776536312848No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCAGCCCTCCTAA30.16759776536312848No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.16759776536312848No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT30.16759776536312848No Hit
TACCCTTACGATGTTCCTGATTACGCACAATTCAGTGCCAGCCCGCCTAA30.16759776536312848No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT30.16759776536312848No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAATGCAGCCCGCCT30.16759776536312848No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCCGCCCGCCTAA30.16759776536312848No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA30.16759776536312848No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA30.16759776536312848No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT30.16759776536312848No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCTCCTAA30.16759776536312848No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT30.16759776536312848No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA30.16759776536312848No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTCA30.16759776536312848No Hit
TACCCTTACGATGTTCCTGATTACGCATAGAGCAAATGCAGCCCGCCTAA30.16759776536312848No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGTATGATACGTGGCATTT20.11173184357541899No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCAGCCCGCCTAA20.11173184357541899No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCTCCTAA20.11173184357541899No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTACATGATACGTGGCATTT20.11173184357541899No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAACCCGCCTAA20.11173184357541899No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG20.11173184357541899No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT20.11173184357541899No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCGCCTAAT20.11173184357541899No Hit
GATGGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATT20.11173184357541899No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT20.11173184357541899No Hit
GATGCGTCCGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT20.11173184357541899No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA20.11173184357541899No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG20.11173184357541899No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAACAGCCCGCCTAATG20.11173184357541899No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT20.11173184357541899No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT20.11173184357541899No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGATTCGTGTTAAAT20.11173184357541899No Hit
TACCCTTACGATGTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAAT20.11173184357541899No Hit
TACCCTTACGATGTTCCTGATTACGCCTAGTGCAAATGCAGCCCGCCTAA20.11173184357541899No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG20.11173184357541899No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT20.11173184357541899No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTTCCAGCCCGCCTAA20.11173184357541899No Hit
GATGCGTCCGCGTCTAACATGTACCCTTTATTGCATGATACGTGGCATTT20.11173184357541899No Hit
GATGAGTCAGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT20.11173184357541899No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT20.11173184357541899No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCCTGATACGTGGCATTT20.11173184357541899No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA20.11173184357541899No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA20.11173184357541899No Hit
TACCCTTACGATGTTCTGATTACGCATAATGTGCAAACAGCCCGCCTAAT20.11173184357541899No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)