FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3D05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3D05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1030
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT27026.21359223300971No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA716.893203883495145No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG565.436893203883495No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG262.524271844660194No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG252.4271844660194173No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG181.7475728155339807No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT151.4563106796116505No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG121.1650485436893203No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG111.0679611650485437No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG100.9708737864077669No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT90.8737864077669903No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA80.7766990291262136No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT80.7766990291262136No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG80.7766990291262136No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG70.6796116504854369No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT70.6796116504854369No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT60.5825242718446602No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC60.5825242718446602No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG60.5825242718446602No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG60.5825242718446602No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA50.48543689320388345No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT40.3883495145631068No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT40.3883495145631068No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA40.3883495145631068No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT40.3883495145631068No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.3883495145631068No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGAC40.3883495145631068No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG40.3883495145631068No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTCAGATTTCGTTGGGGA30.2912621359223301No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.2912621359223301No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.2912621359223301No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT30.2912621359223301No Hit
GATGAGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT30.2912621359223301No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA30.2912621359223301No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT30.2912621359223301No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT30.2912621359223301No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA30.2912621359223301No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.2912621359223301No Hit
TACCCTTACGATGTTCCTGATTACGCTGGCTCGAAGATTTCGTTGGGGAC30.2912621359223301No Hit
GATGAGTCAGCGTCGAACATACAACATTTATTGCATGATACGTGGCATTT30.2912621359223301No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT20.1941747572815534No Hit
GGTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCGTATCGTGGGCTCGAAGATTTCGT20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCGGTGGGCTCGAAGATTTCGT20.1941747572815534No Hit
GATGCGTCCGCGTCTAACATGCACCCTCTATTTCCTGATACGTGTCATTT20.1941747572815534No Hit
GATGAGTCAGCGTCGAACACGCAACATTTATTGCATGATACGTGGCATTT20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA20.1941747572815534No Hit
TACCCTTACGCTGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTT20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAATTTCGTTGGGGACTCTGT20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT20.1941747572815534No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATCGCATGATACGTGGCATTT20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTCGAAGATTTCGTTGGGGAC20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTAGTTTGGGACTCTT20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGT20.1941747572815534No Hit
TACCCTTACGCTGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGTGCTCTAAGATTTCGTT20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGTGGA20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGG20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAATATTTCTTTGGT20.1941747572815534No Hit
GATGCGTCCGCGTCTAACATGCACCCTCTATTGCATGATACGTGGCATTT20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTT20.1941747572815534No Hit
GATGAGTCAGCGTCGAACATGCAACATTCATTGCATGATACGTGGCATTT20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTG20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG20.1941747572815534No Hit
GATGAGTCAGCATCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGTTCGTGGCTCGAAGATTTCGTTGGG20.1941747572815534No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGGCATTT20.1941747572815534No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT20.1941747572815534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)