FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3D01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3D01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1156
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT26823.18339100346021No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG867.439446366782007No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA857.352941176470589No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT242.0761245674740483No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG231.9896193771626298No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG231.9896193771626298No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT191.6435986159169549No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG191.6435986159169549No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG181.5570934256055362No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG141.2110726643598615No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG131.124567474048443No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT121.0380622837370241No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG121.0380622837370241No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG100.8650519031141869No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG90.7785467128027681No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT80.6920415224913495No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT80.6920415224913495No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA80.6920415224913495No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG80.6920415224913495No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT80.6920415224913495No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC70.6055363321799307No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG70.6055363321799307No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG60.5190311418685121No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT60.5190311418685121No Hit
TACCCTTACGATGTTCCTGATTACGCTCCTGGGCTCGAAGATTTCGTTGG60.5190311418685121No Hit
TACCCTTACGATGTTCCTGTGGGCTCGAAGATTTCGTTGGGGACTCTGTC50.43252595155709345No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT50.43252595155709345No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG50.43252595155709345No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT50.43252595155709345No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT50.43252595155709345No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT40.34602076124567477No Hit
TACCCTTACGATGTTCCTGATCTTGGGCTCGAAGATTTCGTTGGGGACTC40.34602076124567477No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT40.34602076124567477No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA40.34602076124567477No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG40.34602076124567477No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA40.34602076124567477No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT30.25951557093425603No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTGGGGACTCTGT30.25951557093425603No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG30.25951557093425603No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA30.25951557093425603No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT30.25951557093425603No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG30.25951557093425603No Hit
TACCCTTACGATGTTCCTGATTACGCTCGTGGGCTCGAAGATTTCGTTGG30.25951557093425603No Hit
GATGAGTCAGCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT30.25951557093425603No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG30.25951557093425603No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG30.25951557093425603No Hit
TACCCTTACGATGTTCCTGATTACGCGTAAATTCCTAGTCGAAGATTTCG30.25951557093425603No Hit
TACCCTTACGATGTTCTGATTACGCTCGAAGATTTCGTTGGGGACTCTGT20.17301038062283738No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTCAGATTTCGTTGGGGA20.17301038062283738No Hit
GATGAGTCCGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT20.17301038062283738No Hit
TACCCTTACGATGTTCCTGCTTACGCTGTGCTCTAAGATTTCGTTGGGGA20.17301038062283738No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG20.17301038062283738No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT20.17301038062283738No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.17301038062283738No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTG20.17301038062283738No Hit
GATGAGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT20.17301038062283738No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGCTCGAAGATTTCGTTGG20.17301038062283738No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT20.17301038062283738No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGCTTTCGTTG20.17301038062283738No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCTAATATTTCTTTGGT20.17301038062283738No Hit
TACCCTTACGATGTTCCTGATTACGCTTCCTGTGCTCGAAGCTTTCGTTG20.17301038062283738No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGTGCTCGAATCTTTCGTTT20.17301038062283738No Hit
TACCCTTACGATGTTCCTGATTACACTGGGCTCGAAGATTTCGTTGGGGA20.17301038062283738No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCTTTGGG20.17301038062283738No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCTTTGGGGACTCT20.17301038062283738No Hit
GATGAGTCCGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT20.17301038062283738No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGTCATTT20.17301038062283738No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGTCTCGAAGATTTCGTTGT20.17301038062283738No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTT20.17301038062283738No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGTCTCGAAGATTTCGTTGG20.17301038062283738No Hit
TACCCTTACGATGTTCCTGATTACGCGCGAAGATTTCGTTGGGGACTCTG20.17301038062283738No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGACTCGAAGATTTCGTTGGGG20.17301038062283738No Hit
TACCCTTACGCTGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT20.17301038062283738No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGAAGATTTCGTTGGGGA20.17301038062283738No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)