FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3C11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3C11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT33224.34017595307918No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA846.158357771260997No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG805.865102639296188No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA332.4193548387096775No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG312.272727272727273No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG302.1994134897360706No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG271.9794721407624634No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG251.8328445747800588No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG241.7595307917888565No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA201.466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT191.3929618768328444No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG191.3929618768328444No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA191.3929618768328444No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT171.2463343108504399No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC151.0997067448680353No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCTGGGCTCGAAGATTTCGTTG141.0263929618768328No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG141.0263929618768328No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA141.0263929618768328No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA130.9530791788856305No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT110.8064516129032258No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT80.5865102639296188No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG70.5131964809384164No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG70.5131964809384164No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA70.5131964809384164No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGAAGATTTCGTTGGGGA70.5131964809384164No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG70.5131964809384164No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT60.43988269794721413No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCATTT60.43988269794721413No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT60.43988269794721413No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGCTCGAAGATTTCGTTGG60.43988269794721413No Hit
TACCCTTACGATGTTCCTGATTACGCCTGTGGGCTCGAAGATTTCGTTGG60.43988269794721413No Hit
TACCCTTACGATGTTCCTGATTACGTTCCGTGGCTCGAAGATTTCGTTGG50.36656891495601174No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA50.36656891495601174No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA50.36656891495601174No Hit
TACCCTTACGATGTTCCTGATTTCGTTGGGGACTCTGTCTCTTATACACA50.36656891495601174No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT50.36656891495601174No Hit
TACCCTTACGATGTTCCTGATTACGCTATGTGGCTCGAAGATTTCGTTGG50.36656891495601174No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT50.36656891495601174No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGTGGGCTCGAAGATTTCGTTG40.2932551319648094No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.2932551319648094No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT40.2932551319648094No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT40.2932551319648094No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT40.2932551319648094No Hit
TACCCTTACGATGTTCCTATTACGCATAAGAGTTTGCCAGCCCGCCTAAT30.21994134897360706No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGCCTCT30.21994134897360706No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.21994134897360706No Hit
GATGAGTCAGCGTCGAACATGCAACATTTAATGCATGATACGTGGCATTT30.21994134897360706No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG30.21994134897360706No Hit
GATGAGTCAGCATCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.21994134897360706No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGCTTTCGTTGGGGACTCTG30.21994134897360706No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA30.21994134897360706No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC30.21994134897360706No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG30.21994134897360706No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.21994134897360706No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA20.1466275659824047No Hit
GATGCGTCCGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT20.1466275659824047No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGACATTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCTGGGCTCGAAGCTTTCGTTG20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGCGCTCGAAGATTTCGTTG20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTTGGCTCGAAGATTTCGTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG20.1466275659824047No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTAGCATTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA20.1466275659824047No Hit
GATGAGTCAGCGTCTAACATGCAACCTTTATTGCATGATACGTGGCATTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGGTG20.1466275659824047No Hit
GATGAGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT20.1466275659824047No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCTCCTAA20.1466275659824047No Hit
ATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTTC20.1466275659824047No Hit
GATGAGTCAGCGTCGAACATGCATCATTTATTGCATGATACGTGGCATTT20.1466275659824047No Hit
TACCCTTACGTTGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTG20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCTAATATTTCTTTGGT20.1466275659824047No Hit
GATGAGTCAGCGTCGAACCTGCAACATTTATTGCATGATACGTGGCATTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGTTTGCTCTAAGATTTCGTT20.1466275659824047No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)