FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3C08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3C08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT24217.19971570717839No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA23716.844349680170577No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA17012.082444918265814No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA765.401563610518834No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG201.4214641080312722No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG181.279317697228145No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG161.1371712864250179No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA120.8528784648187633No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA90.6396588486140725No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA90.6396588486140725No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG80.5685856432125089No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA80.5685856432125089No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGATCGAAGATTTCGTTG70.4975124378109453No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG70.4975124378109453No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA70.4975124378109453No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT70.4975124378109453No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG70.4975124378109453No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA70.4975124378109453No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT60.42643923240938164No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCATCCCGCCTAA60.42643923240938164No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG60.42643923240938164No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG50.35536602700781805No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC50.35536602700781805No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG50.35536602700781805No Hit
TACCCTTACGATGTTCCTGCTTACGCATAGTGCAAATGCAGCCCGCCTAA40.28429282160625446No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT40.28429282160625446No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA40.28429282160625446No Hit
TACCCTTACTATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA40.28429282160625446No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT40.28429282160625446No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG40.28429282160625446No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG40.28429282160625446No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA40.28429282160625446No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCATCCCTCCTAA30.21321961620469082No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA30.21321961620469082No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.21321961620469082No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCTCCTAA30.21321961620469082No Hit
GACGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.21321961620469082No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG30.21321961620469082No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.21321961620469082No Hit
TACCCTTACGATGTTCCTGATTACGCATAGAGCAAATGCAGCCCGCCTAA30.21321961620469082No Hit
GATGAGTCAGCATCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.21321961620469082No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT30.21321961620469082No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCCTGATACGTGTCATTT30.21321961620469082No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT30.21321961620469082No Hit
TACCCTTACGATGTTCCTGATTACGCATGCTTTGCTGCCAGCCCGCCTAA30.21321961620469082No Hit
TACCCTTACGCTGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA30.21321961620469082No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCCAATGCAGCCCGCCTAA20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA20.14214641080312723No Hit
GTTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCGCCTAA20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT20.14214641080312723No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT20.14214641080312723No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCATTT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCATTATTTGCTGCCATCCCTCATAA20.14214641080312723No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTTCTGCCAGCCCGCCTAA20.14214641080312723No Hit
GATGAGTCAGCGTCGAACATGCAACATTTAATGCATGATACGTGGCATTT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGTCTCGAATATTTCGTTGT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCACGATTTGCTGCCAGCCCGCCTAA20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGATCGAAGCTTTCGTTT20.14214641080312723No Hit
GATGAGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCTCCTAA20.14214641080312723No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT20.14214641080312723No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCCTGATACGTGGCATTT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGAATGATTTGCTGCCAGCCCGCCTAA20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGCTGGGGA20.14214641080312723No Hit
TACCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAAT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGACCGCCTAA20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCATGATGTTCTGCCATCCCGCCTAA20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTTCTGCCAGCCCTCCTAA20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAATATTTCGTTGGGGACTCT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA20.14214641080312723No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCTCCTAA20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCGAAGATTTCGTTGGG20.14214641080312723No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGGCATTT20.14214641080312723No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)