FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3C04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3C04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1694
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30818.181818181818183No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA17810.50767414403778No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA1086.375442739079102No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG603.541912632821724No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA422.479338842975207No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT271.5938606847697756No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA251.475796930342385No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG241.4167650531286895No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA231.357733175914994No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA231.357733175914994No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG191.1216056670602124No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG191.1216056670602124No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG171.0035419126328218No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG140.8264462809917356No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG140.8264462809917356No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA130.7674144037780402No Hit
TACCCTTACGATGTTCCTGATTACGCGTAAATTCCTAGTCGAAGATTTCG130.7674144037780402No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT120.7083825265643447No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA120.7083825265643447No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG110.6493506493506493No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT100.5903187721369539No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA90.5312868949232585No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA90.5312868949232585No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG90.5312868949232585No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCGAAGATTTCGTTGGG90.5312868949232585No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGCTCGAAGATTTCGTTGG70.4132231404958678No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT60.3541912632821724No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT60.3541912632821724No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGAAGATTTCGTTGGGGACTC60.3541912632821724No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT60.3541912632821724No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA50.29515938606847697No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGCTCGAAGATTTCGTTGGGG50.29515938606847697No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT50.29515938606847697No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA50.29515938606847697No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT50.29515938606847697No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCTCCTAA50.29515938606847697No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTCAGATTTCGTTGGGGA40.23612750885478156No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGAATCTTTCTTTGGTGACTC40.23612750885478156No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.23612750885478156No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG40.23612750885478156No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCGGGCTCGAAGATTTCGTTGG40.23612750885478156No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC40.23612750885478156No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCTCCTAA40.23612750885478156No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCGCTCAGATTTCGTTGGGGA40.23612750885478156No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG40.23612750885478156No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG40.23612750885478156No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCGCCTAA30.1770956316410862No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.1770956316410862No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG30.1770956316410862No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCGCCTAA30.1770956316410862No Hit
TACCCTTACGATGTTCCTGATTACTGGGCTCGAAGATTTCGTTGGGGACT30.1770956316410862No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.1770956316410862No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT30.1770956316410862No Hit
TACCCTTACGCTGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT30.1770956316410862No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT30.1770956316410862No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTT30.1770956316410862No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG30.1770956316410862No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG30.1770956316410862No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT30.1770956316410862No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTTGCATTT30.1770956316410862No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT30.1770956316410862No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGTCTCGAATATTTCGTTGT30.1770956316410862No Hit
GAGGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCTTTGGT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGCAAATGCAGCCCGCCTAA20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGACTCTGT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT20.11806375442739078No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCATTT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCGGGCTCGAAGATTTCGTTGT20.11806375442739078No Hit
TACCCTTACTATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA20.11806375442739078No Hit
GATAAGTCAGCGTCGAACATGCAACATTTATTACATGATACGTGGCATTT20.11806375442739078No Hit
TACCCTTACGATTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGAC20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCATCCCTCCTAA20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTGGGGACTCTT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGTCTCGAAAATTTCGTTGT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGGTTTCGTTT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCTCCTAA20.11806375442739078No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTTCTGCCAGCCCGCCTAA20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGT20.11806375442739078No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGTCATTT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG20.11806375442739078No Hit
TACCCTCGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAATG20.11806375442739078No Hit
GATGAGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT20.11806375442739078No Hit
GATGCGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCTCCTAA20.11806375442739078No Hit
GATGAGTCAGAGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGCTTACGCATGAGCCTGGAACAGCCCGCCTAA20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCATCATTCAGTGCCAGCCCGCCTAA20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT20.11806375442739078No Hit
GATGAGTCAGCGTCGAACATGCAACATTTAGTGCATGATACGTGGCATTT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGTCTCGAATATTTCGTTGT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGAGGACTCTG20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTAGTTTGGGACTCTT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACTGTGCTCGAAGATTTCGTTGTGGACT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA20.11806375442739078No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACATGGCATTT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCTCCTAA20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAATATTTCGTTGG20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCGCCGTGTCTCGAAGATTTCGTTGT20.11806375442739078No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGT20.11806375442739078No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGGCATTT20.11806375442739078No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA20.11806375442739078No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)