FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3C01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3C01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT31622.051639916259596No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG966.699232379623169No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA664.6057222609909285No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG292.0237264480111654No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA281.9539427773900906No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG281.9539427773900906No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG251.7445917655268668No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT231.6050244242847174No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT231.6050244242847174No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA211.465457083042568No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG181.2561060711793441No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG171.1863224005582695No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG161.1165387299371947No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT161.1165387299371947No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA161.1165387299371947No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGCTCGAAGATTTCGTTGGGG130.9071877180739707No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCTGGGCTCGAAGATTTCGTTG130.9071877180739707No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG110.7676203768318213No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG110.7676203768318213No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG100.6978367062107467No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT100.6978367062107467No Hit
TACCCTTACGATGTTCCTGATTGCTCGAAGATTTCGTTGGGGACTCTGTC90.6280530355896721No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT80.5582693649685974No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG80.5582693649685974No Hit
TACCCTTACGATGTTCCTGATTACGCTGGCTCGAAGATTTCGTTGGGGAC80.5582693649685974No Hit
TACCCTTACGATGTTCCTGATTACGGTCTTGGGCTCGAAGATTTCGTTGG80.5582693649685974No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT70.48848569434752265No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGCTCGAAGATTTCGTTGG70.48848569434752265No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA70.48848569434752265No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG70.48848569434752265No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT60.41870202372644805No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG60.41870202372644805No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT50.34891835310537334No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT50.34891835310537334No Hit
TACCCTTACGATGTTCCTGATTACGCAGTGGGCTCGAAGATTTCGTTGGG50.34891835310537334No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA50.34891835310537334No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGATCGAAGATTTCGTTGGGGA40.2791346824842987No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT40.2791346824842987No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATAATACGTGGCATTT40.2791346824842987No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA40.2791346824842987No Hit
TACCCTTACGATGTTCCTGATTACGCTCCTGGGCTCGAAGATTTCGTTGG40.2791346824842987No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.2791346824842987No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT40.2791346824842987No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT30.20935101186322402No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT30.20935101186322402No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG30.20935101186322402No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA30.20935101186322402No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT30.20935101186322402No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG30.20935101186322402No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA30.20935101186322402No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT30.20935101186322402No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTTGTTGG30.20935101186322402No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG30.20935101186322402No Hit
TACCCTTACGCTGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT30.20935101186322402No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGGCATTT30.20935101186322402No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA20.13956734124214934No Hit
GAGGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.13956734124214934No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCTGGTCTCGAATCTTTCGTTT20.13956734124214934No Hit
GATGCGTCCGCGTCTAACATGCACCCTCTATTGCCTGATACGTGGCATTT20.13956734124214934No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA20.13956734124214934No Hit
TACCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTGT20.13956734124214934No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATCT20.13956734124214934No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.13956734124214934No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT20.13956734124214934No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGCTCGAAGATTTCGTTGGGT20.13956734124214934No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT20.13956734124214934No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGGTG20.13956734124214934No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTG20.13956734124214934No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT20.13956734124214934No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT20.13956734124214934No Hit
TACCCTTACGATGTTCCTGATTACGCTCCTGGGCTCGAAGATTTCGTTGT20.13956734124214934No Hit
GATGAGTAAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.13956734124214934No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTTTCTCGAAACTTTCGGTT20.13956734124214934No Hit
GCTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.13956734124214934No Hit
TACCCTTACGATGTTCCTGATTACGCTCGTGGGCTCGAAGATTTCGTTGG20.13956734124214934No Hit
GATGAGTCAGCGTAGAACATGCAACATTTATTGCATGATACGTGGCATTT20.13956734124214934No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG20.13956734124214934No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTG20.13956734124214934No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTT20.13956734124214934No Hit
GATGAGTCCGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT20.13956734124214934No Hit
GATGAGTCCGCGTCTAACATGCACCATTTATTGCATGATACGTGGCATTT20.13956734124214934No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA20.13956734124214934No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT20.13956734124214934No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGTCTCGAAGATTTCGTT20.13956734124214934No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)