FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3B12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3B12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1551
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA30319.535783365570598No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT28018.052869116698904No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA1056.769825918762089No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA744.771115409413282No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA603.8684719535783367No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA533.4171502256608637No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG332.127659574468085No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA241.5473887814313347No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG191.2250161186331399No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG171.096067053513862No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG140.9026434558349452No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG130.8381689232753062No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG130.8381689232753062No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA120.7736943907156674No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT110.7092198581560284No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG100.6447453255963894No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG90.5802707930367506No Hit
TACCCTTACGATGTTCCTGATTACGCTTAAGCGGCCGCAAAAGGAAAATC90.5802707930367506No Hit
TACCCTTACGATGTTCCTGATTACGCATAGAGCAAATGCAGCCCGCCTAA90.5802707930367506No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCGCCTAA70.4513217279174726No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA70.4513217279174726No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT60.3868471953578337No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT60.3868471953578337No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT50.3223726627981947No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG50.3223726627981947No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT50.3223726627981947No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG50.3223726627981947No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA40.2578981302385558No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.2578981302385558No Hit
TACCCTTACTATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA40.2578981302385558No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCGCCTAA40.2578981302385558No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAATGCAGCCCGCCT40.2578981302385558No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC40.2578981302385558No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGCTCGAAGATTTCGTTGG40.2578981302385558No Hit
GATGAGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT40.2578981302385558No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT30.19342359767891684No Hit
TACCCTTACGATGTTCCTGATTACGAAGATTTCGTTGGGGACTCTGTCTC30.19342359767891684No Hit
TACCCTTACGATGTTCCTGATTACGCATAAATCAGTGCCAGCCCGCCTAA30.19342359767891684No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAG30.19342359767891684No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT30.19342359767891684No Hit
TACCCTTACGATGTTCCTGATTACGCAGTGGGCTCGAAGATTTCGTTGGG30.19342359767891684No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG30.19342359767891684No Hit
GATGAGTCAGCGTCGAACATGCAACATTTAATGCATGATACGTGGCATTT30.19342359767891684No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCTCCTAA30.19342359767891684No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT30.19342359767891684No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT30.19342359767891684No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCATCCCTCCTAA30.19342359767891684No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAGCAGCCCGCCTAA30.19342359767891684No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG30.19342359767891684No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCAGGATACGTGGCATTT30.19342359767891684No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA30.19342359767891684No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCAGCCCGCCTAA20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCACAAGAGTTTGCCAGCCCGCCTAA20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCATAGAGCACATGCAGCCCGCCTAA20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCACATGCAGCCCGCCTAA20.1289490651192779No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCCTGATACGTGTCATTT20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGATCGAAGATTTCGTTG20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTCA20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGACCGCCTAA20.1289490651192779No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCACGATACGTGGCATTT20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA20.1289490651192779No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATTCGTGGCATTT20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGG20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACTGGGCTCGAAGATTTCGTTGGGGACT20.1289490651192779No Hit
GATAAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1289490651192779No Hit
ACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAAT20.1289490651192779No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGTTACGTGGCATTT20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCTCCTAA20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCATCCCGCCTAA20.1289490651192779No Hit
TACCCTTACGATGTTCTGATTACGCATAGTGCAAATGCAGCCCGCCTAAT20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGCTTTCGTTG20.1289490651192779No Hit
TACCCTTACTATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA20.1289490651192779No Hit
TACCCTTACGATGTCCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA20.1289490651192779No Hit
TACCCTTACTATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTCA20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCGCGCCTAA20.1289490651192779No Hit
TCCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAAT20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCGTTGGG20.1289490651192779No Hit
GATGAGTCAGCGTCGACCATGCAACATTTATTGCATGATACGTGGCATTT20.1289490651192779No Hit
GATGATTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1289490651192779No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT20.1289490651192779No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)