FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3B08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3B08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407
Sequences flagged as poor quality0
Sequence length301
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT22716.13361762615494No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA1057.462686567164178No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG896.325515280739161No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA563.9800995024875623No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA362.55863539445629No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG342.4164889836531627No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA322.2743425728500357No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG302.1321961620469083No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA281.9900497512437811No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG271.9189765458422177No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA241.7057569296375266No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG231.634683724235963No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG201.4214641080312722No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG191.3503909026297085No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA181.279317697228145No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA181.279317697228145No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG151.0660980810234542No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT120.8528784648187633No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG120.8528784648187633No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA120.8528784648187633No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA110.7818052594171997No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG90.6396588486140725No Hit
GATGAGTCAGCGTCGAACATGCAACATTTAATGCATGATACGTGGCATTT50.35536602700781805No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT50.35536602700781805No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG50.35536602700781805No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT40.28429282160625446No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.28429282160625446No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.28429282160625446No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT40.28429282160625446No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT40.28429282160625446No Hit
TACCCTTACGATGTTCCTGATTACGCAGTGGGCTCGAAGATTTCGTTGGG40.28429282160625446No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG40.28429282160625446No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT40.28429282160625446No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCGCCTAA30.21321961620469082No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT30.21321961620469082No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT30.21321961620469082No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG30.21321961620469082No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG30.21321961620469082No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA30.21321961620469082No Hit
TACCCTTACGATGTTCCTGATTACGCGTTCGGGGCTCGAAGATTTCGTTG30.21321961620469082No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG30.21321961620469082No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA30.21321961620469082No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT30.21321961620469082No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.21321961620469082No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT30.21321961620469082No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG30.21321961620469082No Hit
TACCCTTACGATGTTCCTGATTACGCTAGAAGATTTCGTTGGGGACTCTG20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGTAACAGCCCGCCTAA20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTCAGATTTCGTTGGGGA20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA20.14214641080312723No Hit
TACCCATACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGGTGGGGA20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGTCTCGAAAATTTCGTTGT20.14214641080312723No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA20.14214641080312723No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT20.14214641080312723No Hit
GATGAGTCAGCGTCGAACATGCAACACTTATTGCATGATACGTGGCATTT20.14214641080312723No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGGGTCGAAGATTTCGTTGGGGACTCT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTTG20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTG20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGCTTTCGTTGGGGACTCTG20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGTCTCGAAGATTTCGTTGG20.14214641080312723No Hit
GATGCGTCCGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT20.14214641080312723No Hit
TACCCTTACTATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAATATTTCGTTGG20.14214641080312723No Hit
TACCCTTACTATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCATATGAGTTTGCCAGCCCGCCTAA20.14214641080312723No Hit
GATGAGTCAGCGTCGAACATGAAACATTTATTGCATGATACGTGGCATTT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGT20.14214641080312723No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGTCTCGAATATTTCGTTGT20.14214641080312723No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)