FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3B06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3B06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1146
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT24721.55322862129145No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA746.457242582897033No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA736.369982547993019No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA716.195462478184991No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA625.4101221640488655No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA494.275741710296685No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA413.5776614310645725No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG383.315881326352531No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA282.4432809773123907No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG211.832460732984293No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG191.6579406631762654No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG191.6579406631762654No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTTCGA121.0471204188481675No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG90.7853403141361256No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG80.6980802792321117No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCGAAGATTTCGTTGGG80.6980802792321117No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT70.6108202443280977No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG70.6108202443280977No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT60.5235602094240838No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA60.5235602094240838No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT50.4363001745200698No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT50.4363001745200698No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT50.4363001745200698No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT50.4363001745200698No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA40.34904013961605584No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.34904013961605584No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT40.34904013961605584No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA40.34904013961605584No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG30.2617801047120419No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT30.2617801047120419No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.2617801047120419No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT30.2617801047120419No Hit
GATGAGTCAGCGTTGAACATGCAACATTTATTGCATGATACGTGGCATTT30.2617801047120419No Hit
TACCCTTACTATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA30.2617801047120419No Hit
TACCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAAT30.2617801047120419No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCTCCTAA20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGCACAAGAGTTTGCCAGCCCGCCTAA20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGCTCCTGGGCTCGAAGATTTCGTTGG20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCTCCTAA20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGCATTAGCCTGGAACAGCCCGCCTAA20.17452006980802792No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGTTGTGGGCTCGAAGATTTCGTTGGG20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG20.17452006980802792No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGCACGATTTGCTGCCAGCCCGCCTAA20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCTCCTAA20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCTCCTAA20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGCTCGAAGATTTCGTTGGGG20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGT20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG20.17452006980802792No Hit
GATGAGTCAGCGTCGAACATGCAACATTAATTGCATGATACGTGGCATTT20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGCATAGAGCAAATGCAGCCCGCCTAA20.17452006980802792No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG20.17452006980802792No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)