FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3B05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3B05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1001
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT24124.07592407592408No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA565.594405594405594No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG313.096903096903097No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG282.797202797202797No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT272.697302697302697No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG161.5984015984015985No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG151.4985014985014986No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG141.3986013986013985No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG131.2987012987012987No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA121.1988011988011988No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG121.1988011988011988No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG100.999000999000999No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG100.999000999000999No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT90.8991008991008992No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT90.8991008991008992No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT80.7992007992007992No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA80.7992007992007992No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG80.7992007992007992No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT70.6993006993006993No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG70.6993006993006993No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT60.5994005994005994No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG60.5994005994005994No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT50.4995004995004995No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT50.4995004995004995No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT50.4995004995004995No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT50.4995004995004995No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG50.4995004995004995No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGCTCGAAGATTTCGTTGG50.4995004995004995No Hit
TACCCTTACGATGTTCCTGATTACGGCTGTGGGCTCGAAGATTTCGTTGG50.4995004995004995No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA50.4995004995004995No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT40.3996003996003996No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.3996003996003996No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTAGCATTT40.3996003996003996No Hit
GATGAGTCAGCGTCAAACATGCAACATTTATTGCATGATACGTGGCATTT40.3996003996003996No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT30.2997002997002997No Hit
TACCCTTACGATGTTCCTGATTACGCTCGTGGGCTCGAAGATTTCGTTGG30.2997002997002997No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT30.2997002997002997No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA30.2997002997002997No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGTGGGCTCGAAGATTTCG30.2997002997002997No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG30.2997002997002997No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG30.2997002997002997No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT30.2997002997002997No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGTATGATACGTGGCATTT20.1998001998001998No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT20.1998001998001998No Hit
GATGAGTCAGCATCGAACATGCAACATTTATTGCATGATACATGACATTT20.1998001998001998No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTACATGATACGTGGCATTT20.1998001998001998No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCCTGATACGTGGCATTT20.1998001998001998No Hit
GATGAGTCAGCGTCTAACATGCACCATTTATTGCATGATACGTGGCATTT20.1998001998001998No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT20.1998001998001998No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT20.1998001998001998No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT20.1998001998001998No Hit
TACCCTTACGATGTTCCTGATTACGCCTGTGGGCTCGAAGATTTCGTTGG20.1998001998001998No Hit
GATGAGTCAGCGTAGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1998001998001998No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG20.1998001998001998No Hit
GATGAGTCCGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT20.1998001998001998No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG20.1998001998001998No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGGAGATTTCGTTG20.1998001998001998No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGCTCGAAGATTTCGTTG20.1998001998001998No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG20.1998001998001998No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACATGGCATTT20.1998001998001998No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGTTCTCGAATCTTTCGTTT20.1998001998001998No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT20.1998001998001998No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTGCTTTGGT20.1998001998001998No Hit
GATGCGTCCGCGTCTAACATGAACCCTTTATTTCCTGATACGTGTCATTT20.1998001998001998No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG20.1998001998001998No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTGTTTGCTCTAAGATTTCGTT20.1998001998001998No Hit
TACCCTTACGATGTTCCTGATTACGGTGTGCTCTCAGATTTCGTTGGGGA20.1998001998001998No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG20.1998001998001998No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTGGGGAC20.1998001998001998No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)