FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3B04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3B04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1550
Sequences flagged as poor quality0
Sequence length301
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT23315.03225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA885.67741935483871No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA855.483870967741936No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA835.35483870967742No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA634.064516129032258No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA573.6774193548387095No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA553.5483870967741935No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA503.225806451612903No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG452.903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG322.064516129032258No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG261.6774193548387095No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG150.967741935483871No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG150.967741935483871No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG110.7096774193548387No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA110.7096774193548387No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT100.6451612903225806No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA100.6451612903225806No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG90.5806451612903226No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT70.4516129032258065No Hit
TACCCTTACGATGTTCCTGATTGTGGGCTCGAAGATTTCGTTGGGGACTC70.4516129032258065No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT60.3870967741935484No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT60.3870967741935484No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG60.3870967741935484No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGAAGATTTCGTTGGGGA60.3870967741935484No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG60.3870967741935484No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG50.3225806451612903No Hit
TACCCTTACGATGTTCCTGATGGGCTCGAAGATTTCGTTGGGGACTCTGT50.3225806451612903No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT50.3225806451612903No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG50.3225806451612903No Hit
TACCCTTACGATGTTCCTGATTACGCGTAAATTCCTAGTCGAAGATTTCG50.3225806451612903No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT40.25806451612903225No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG40.25806451612903225No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGGCTCTAATATTTCTTTGGT40.25806451612903225No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT40.25806451612903225No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG30.1935483870967742No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAATGCAGCCCGCCT30.1935483870967742No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCGCTCAGATTTCGTTGGGGA30.1935483870967742No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT30.1935483870967742No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG30.1935483870967742No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCTCCTAA30.1935483870967742No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGCTCGAAGATTTCGTTGG30.1935483870967742No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT30.1935483870967742No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA30.1935483870967742No Hit
TACCCTTACGATGTTCCTGATTACGCCTGTGGGCTCGAAGATTTCGTTGG30.1935483870967742No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT30.1935483870967742No Hit
TACCCTTACGATGTTCCTGATTACGTTCGGGCTCGAAGATTTCGTTGGGG30.1935483870967742No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA30.1935483870967742No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCTAATATTTCTTTGGT30.1935483870967742No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTT30.1935483870967742No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG30.1935483870967742No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCTCCTAA30.1935483870967742No Hit
GATGAGTCAGCGTCGAACATGCACCATCTATTGCATGATACGTGGCATTT20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCATGTGCCTGGAACAGCCCGCCTAA20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGTAACAGCCCGCCTAA20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCACAAGAGTTTGCCAGCCCGCCTAA20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGAGTCTGTGGGCTCGAAGATTTCGTT20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTCAGATTTCGTTGGGGA20.12903225806451613No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTATGGCTCGAAGATTTCGTTG20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCTCCTAA20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGAGCTCGAAGATTTCGTTGGG20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGAGTTCGTTG20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCATTAGCCTGGAACAGCCCGCCTAA20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCTCCTAA20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAACT20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCATCCCGCCTAA20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCACGATTTGCTGCCAGCCCGCCTAA20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCGTAAATTCCTAGTCGCAGATTTCG20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT20.12903225806451613No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGG20.12903225806451613No Hit
GATGAGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGCTCGAAGATTTCGTTGGGT20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCTCCTAA20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA20.12903225806451613No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGCAGATTTCGTTGGGGA20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCTCATAA20.12903225806451613No Hit
GATGAGTCATCGTCGCACATGCAACATTTATTGCATGATACGTGGCATTT20.12903225806451613No Hit
GATGAGTCAGCGTAGAACATGCAACATTTATTGCATGATACGTGGCATTT20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGAAGATTTCGTTGGGGACTC20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTCGTTGGGGAC20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT20.12903225806451613No Hit
GATGCGTCCGCGTCTAACATGAACCCTTTATGTCCTGATACGTGTCATTT20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTT20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCTGGCGAAGATTTCGTTGGGGACTC20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGGAGATTTCGTTG20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAACAGCCCGCCTAATG20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGT20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACAGCCCTCCTAA20.12903225806451613No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACATGGCATTT20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCGTTGGT20.12903225806451613No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGTCTCGAATATTTCGTTGT20.12903225806451613No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA20.12903225806451613No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)