FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3B02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3B02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1684
Sequences flagged as poor quality0
Sequence length301
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT26615.795724465558195No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA17510.391923990498812No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA1156.828978622327791No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA1005.938242280285035No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA905.344418052256532No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA764.513064133016627No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG663.9192399049881232No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA593.503562945368171No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA583.44418052256532No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG251.4845605700712587No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG241.4251781472684086No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT160.9501187648456058No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG150.8907363420427553No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG140.831353919239905No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG100.5938242280285035No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG90.5344418052256532No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG80.4750593824228029No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCGAAGATTTCGTTGGG80.4750593824228029No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG70.4156769596199525No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC60.35629453681710216No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT60.35629453681710216No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA60.35629453681710216No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCATCCCGCCTAA50.29691211401425177No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT50.29691211401425177No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCATCCCGCCTAA50.29691211401425177No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA50.29691211401425177No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG50.29691211401425177No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG50.29691211401425177No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA50.29691211401425177No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCGCCTAA40.23752969121140144No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.23752969121140144No Hit
TACCCTTACGATGTTCCTGATTACGCATAGAGCAAATGCAGCCCGCCTAA40.23752969121140144No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG40.23752969121140144No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT40.23752969121140144No Hit
TACCCTTACGATGTTCCTGATTACGCACGAGCCTGGAACAGCCCGCCTAA40.23752969121140144No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG40.23752969121140144No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT30.17814726840855108No Hit
TACCCTTACGATGTTCCTGATTACGCACAAGAGTTTGCCAGCCCGCCTAA30.17814726840855108No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT30.17814726840855108No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA30.17814726840855108No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA30.17814726840855108No Hit
TACCCTTACGATGTTCCTGATTACGCACAGTGCAAATGCAGCCCGCCTAA30.17814726840855108No Hit
TACCCTTACGATGTTCCTGATTACGCACGATTTGCTGCCAGCCCGCCTAA30.17814726840855108No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT30.17814726840855108No Hit
TACCCTTACGATGTTCCTGATTACGCACAATTCAGTGCCAGCCCGCCTAA30.17814726840855108No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAACAGCCCGCCTAATG30.17814726840855108No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.17814726840855108No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCTCCTAA30.17814726840855108No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGT30.17814726840855108No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCCAATGCAGCCCGCCTAA20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGTAACAGCCCGCCTAA20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGCTTTCGTTG20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCCAA20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCTCCTAA20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCACAATTCAGTGCCATCCCGCCTAA20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT20.11876484560570072No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCATTT20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG20.11876484560570072No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCACATGCATCCCGCCTAA20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCATCCCTCCTAA20.11876484560570072No Hit
TACCCTTACGATGTGCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG20.11876484560570072No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT20.11876484560570072No Hit
GATGAGTCAGCGTCGAACATGCAACATTTAATGCATGATACGTGGCATTT20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCATTAGCCTGTAACAGCCCGCCTAA20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCTCCTAA20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGGAGATTTCGTTG20.11876484560570072No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCATCCCTCCTAA20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCATAGGGCAAATGCAGCCCGCCTAA20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCTCCTAA20.11876484560570072No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATTCAGTGCCATCCCTCCTAA20.11876484560570072No Hit
GATGAGTCAGCGTCTAACATGCACCATTTATTGCATGATACGTGGCATTT20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCATCATTCAGTGCCAGCCCGCCTAA20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCTCCTAA20.11876484560570072No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGTCATTT20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACAGCCCTCCTAA20.11876484560570072No Hit
GATGAGTTAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.11876484560570072No Hit
TACCCTTACGCTGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT20.11876484560570072No Hit
TACCCTTACGCTGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCATCCCTCCTAA20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTCA20.11876484560570072No Hit
TACCCTTACGATGTTCCTGATTACGCGTAAATTCCTAGTCGAAGATTTCG20.11876484560570072No Hit
TACCCTTACGCTGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA20.11876484560570072No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)