FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3B01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3B01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41
Sequences flagged as poor quality0
Sequence length301
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1536.58536585365854No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT1024.390243902439025No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA37.317073170731707No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG12.4390243902439024No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA12.4390243902439024No Hit
GAGGCGTCCGCGTCTAACATGTACCCTTTATGTCCTGATACGTGTCATTT12.4390243902439024No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT12.4390243902439024No Hit
GAGGAGTCCGCGTCTAACATGCACCCTTTATTTCCTGATACGTGTCATTT12.4390243902439024No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA12.4390243902439024No Hit
GATGAGTCCGCGTCAAACATGCACCATTTATTGCATGATACGTGGCATTT12.4390243902439024No Hit
TACCCTTACGATGTTCCTGATTACGCTTCTTGTTCTCGAAGCTTTCGTTT12.4390243902439024No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCCCGAAGATTTCGTTGGGT12.4390243902439024No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCTTTGGT12.4390243902439024No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG12.4390243902439024No Hit
TACCCTTACGATGTTCCTGATTACGTCCTTGCGCAACGGTTTCGTAGGCG12.4390243902439024No Hit
GAGGAGTCAGCGTCGAACATGCAACATTTATTACCTGATACGTGGCATTT12.4390243902439024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)