FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3A11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3A11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1568
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT23514.987244897959185No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA1308.290816326530612No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA1268.035714285714286No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA1177.461734693877552No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA855.420918367346939No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA563.571428571428571No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG442.806122448979592No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA422.6785714285714284No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG382.423469387755102No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA311.9770408163265307No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG181.1479591836734695No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG171.0841836734693877No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG110.701530612244898No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG100.6377551020408163No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG90.5739795918367347No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA90.5739795918367347No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG80.5102040816326531No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG70.4464285714285714No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT60.3826530612244898No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG60.3826530612244898No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT60.3826530612244898No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT60.3826530612244898No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT50.31887755102040816No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT50.31887755102040816No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA50.31887755102040816No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG50.31887755102040816No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG50.31887755102040816No Hit
TACCCTTACTATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA50.31887755102040816No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGGCATTT50.31887755102040816No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCATCCCGCCTAA40.25510204081632654No Hit
GATGAGTCAGCGTCGAACATGCAACATTTAATGCATGATACGTGGCATTT40.25510204081632654No Hit
TACCCTTACGATGTTCCTGATTACGCATAGAGCAAATGCAGCCCGCCTAA40.25510204081632654No Hit
TACCCTTACGATGTTCCTGCTTACGCATAAGAGTTTGCCAGCCCGCCTAA40.25510204081632654No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA40.25510204081632654No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA40.25510204081632654No Hit
TACCCTTACGATGTTCCTGATTACGCACAAGAGTTTGCCAGCCCGCCTAA30.1913265306122449No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT30.1913265306122449No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT30.1913265306122449No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATAATACGTGGCATTT30.1913265306122449No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGGTTGCCAGCCCGCCTAA30.1913265306122449No Hit
GATGCGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT30.1913265306122449No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCTGGGCTCGAAGATTTCGTTG30.1913265306122449No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA30.1913265306122449No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT30.1913265306122449No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCTCCTAA30.1913265306122449No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC30.1913265306122449No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG30.1913265306122449No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT30.1913265306122449No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCTCCTAA30.1913265306122449No Hit
TCCCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA30.1913265306122449No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA20.12755102040816327No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCATCCCGCCTAA20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAATGCAGCCCGCCT20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGACTCGAAGATTTCGTTGGGG20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCATCCCTCCTAA20.12755102040816327No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT20.12755102040816327No Hit
TACCCTTACGATGTTCCCGATTACGCATAATTCAGTGCCAGCCCGCCTAA20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCTCCTAA20.12755102040816327No Hit
GATGAGTCAGCATCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTCTGCCAGCCCGCCTAA20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGCAGATTTCGTTGGGGACTCT20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCTCCTAA20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATCTTGGGCTCGAAGATTTCGTTGGGGACTC20.12755102040816327No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCACGATACGTGGCATTT20.12755102040816327No Hit
GATGCGTCCGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTACGCACAATTCAGTGCCAGCCCGCCTAA20.12755102040816327No Hit
TACCCTTACTATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGAAGATTTCGTTGGGGACTC20.12755102040816327No Hit
TCCCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA20.12755102040816327No Hit
TACCCTTACGCTGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA20.12755102040816327No Hit
GATGCGTCCGCGTCTAACATGAACCCTTTATGTCCTGATACGTGTCATTT20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGACTCTG20.12755102040816327No Hit
GATGAGTCAGCGTCGATCATGCAACATTTATTGCATGATACGTGGCATTT20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGTGCTCTAAGATTTCGTT20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTGTGGGCTCGAAGATTTCGTTGGGGACTC20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGAAGATTTCGTTGGGGA20.12755102040816327No Hit
TACCCTTACTATGTTCCTGATTACGCATAGTTCAAATGCAGCCCGCCTCA20.12755102040816327No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTCA20.12755102040816327No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)