FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3A10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3A10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1078
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT22120.500927643784784No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA14313.26530612244898No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG12111.224489795918368No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG363.339517625231911No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG252.3191094619666046No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG232.1335807050092765No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG141.2987012987012987No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC121.1131725417439702No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT111.0204081632653061No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA111.0204081632653061No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT100.927643784786642No Hit
TACCCTTACGATGTTCCTGATTACGCCCGGGCTCGAAGATTTCGTTGGGG90.8348794063079777No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG90.8348794063079777No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG90.8348794063079777No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG90.8348794063079777No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA90.8348794063079777No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT70.6493506493506493No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG70.6493506493506493No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG60.5565862708719851No Hit
TACCCTTACGATGTTCCTGATTTCGTTGGGGACTCTGTCTCTTATACACA50.463821892393321No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA50.463821892393321No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGGGCTCGAAGATTTCGTTGGG50.463821892393321No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT50.463821892393321No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT40.3710575139146568No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA40.3710575139146568No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.3710575139146568No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA40.3710575139146568No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.3710575139146568No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA40.3710575139146568No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG40.3710575139146568No Hit
GATGCGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT30.27829313543599254No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCCTGATACGTGTCATTT30.27829313543599254No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT30.27829313543599254No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.27829313543599254No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT30.27829313543599254No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA30.27829313543599254No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTG30.27829313543599254No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG30.27829313543599254No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA30.27829313543599254No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTTCATGATACGTGGCATTT30.27829313543599254No Hit
TACCCTTACGACGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG20.1855287569573284No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA20.1855287569573284No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTG20.1855287569573284No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTAACATTT20.1855287569573284No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG20.1855287569573284No Hit
TACCCTTACTGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGACT20.1855287569573284No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATCGCATGATACGTGGCATTT20.1855287569573284No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG20.1855287569573284No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGATCTGT20.1855287569573284No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGGAGATTTCGTTG20.1855287569573284No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC20.1855287569573284No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCGCGCAGATTTCGTTGGGGA20.1855287569573284No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT20.1855287569573284No Hit
GATGAGTCAGCGTCAAACATGCAACATTTATTACATAATACGTGGCATTT20.1855287569573284No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT20.1855287569573284No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGAC20.1855287569573284No Hit
TACCCTTACGATGTTCCTGATTACTCGAAGATTTCGTTGGGGACTCTGTC20.1855287569573284No Hit
GATGAGTCAGCGTCGAACATGCACCCTTTATTGCATGATACGTGGCATTT20.1855287569573284No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT20.1855287569573284No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)