FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3A05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3A05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1040
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT19418.653846153846153No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA565.384615384615385No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT484.615384615384616No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG464.423076923076923No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG444.230769230769231No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG242.307692307692308No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG191.826923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG181.7307692307692308No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG161.5384615384615385No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG131.25No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG121.153846153846154No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG121.153846153846154No Hit
TACCCTTACGATGTTCCTGATTACGCCTGTGGGCTCGAAGATTTCGTTGG100.9615384615384616No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC80.7692307692307693No Hit
TACCCTTACGATGTTCCTGATTACGCAGTGGGCTCGAAGATTTCGTTGGG80.7692307692307693No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGCTCGAAGATTTCGTTGG80.7692307692307693No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT60.576923076923077No Hit
TACCCTTACGATGTTCCTGATTAGTGGGCTCGAAGATTTCGTTGGGGACT60.576923076923077No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG60.576923076923077No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT50.4807692307692308No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG50.4807692307692308No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT50.4807692307692308No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG40.38461538461538464No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT40.38461538461538464No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGGGCTCGAAGATTTCGTTGGG40.38461538461538464No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT40.38461538461538464No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG40.38461538461538464No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG40.38461538461538464No Hit
TACCCTTACGATGTTCCTGATTACGTTCGTGGCTCGAAGATTTCGTTGGG40.38461538461538464No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA30.2884615384615385No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGTGGGCTCGAAGATTTCGTTG30.2884615384615385No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCTTTGGT30.2884615384615385No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT30.2884615384615385No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT30.2884615384615385No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCATTT30.2884615384615385No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG30.2884615384615385No Hit
GATGAGTCAGCGTCGAACATGCAACCTTTATTGCATGATACGTGGCATTT30.2884615384615385No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGGTG30.2884615384615385No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT30.2884615384615385No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA30.2884615384615385No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC30.2884615384615385No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG30.2884615384615385No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT30.2884615384615385No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA30.2884615384615385No Hit
TACCCTTACGATGTTCTGATTACGCTCGAAGATTTCGTTGGGGACTCTGT20.19230769230769232No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT20.19230769230769232No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA20.19230769230769232No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.19230769230769232No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTATGGCTCGAAGATTTCGTTG20.19230769230769232No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT20.19230769230769232No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG20.19230769230769232No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCGCTCAGATTTCGTTGGGGA20.19230769230769232No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGTCTCGAATATTGCGTGGT20.19230769230769232No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATAT20.19230769230769232No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT20.19230769230769232No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT20.19230769230769232No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGAGGGCTCGAAGATTTCGTTG20.19230769230769232No Hit
TACCCTTACGATGTTCCTGATTACGCAGTGGGCTCGAAGATTTCGTTGGT20.19230769230769232No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGTGCTCGAAGCTTTCGTTT20.19230769230769232No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCGTTT20.19230769230769232No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT20.19230769230769232No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCCTGATACGTGTCATTT20.19230769230769232No Hit
TACCCTTACGATGTTCCTGATAGCGTCTAGCCAGCCGATTCGTGTTAAAT20.19230769230769232No Hit
TACCCTTACGCTGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG20.19230769230769232No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT20.19230769230769232No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCTAATATTTCTTTGGG20.19230769230769232No Hit
GATGAGTCAGCGTCGATTCGGCGCTGGTCTATCGCGGCATGGACATCGGC20.19230769230769232No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT20.19230769230769232No Hit
GATGAGTCAGCGTCGAACATGCAACATTTACTGCATGATACGTGGCATTT20.19230769230769232No Hit
GATGCGTCCGCGTCTAACATGCACCATTTATTGCATGATACGTGGCATTT20.19230769230769232No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGCTCGAAGCTTTCGTTG20.19230769230769232No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)