FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3A04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3A04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1364
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT24117.668621700879765No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA1007.331378299120235No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG896.524926686217008No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT463.3724340175953076No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG322.346041055718475No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG241.7595307917888565No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG221.6129032258064515No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG191.3929618768328444No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG191.3929618768328444No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG141.0263929618768328No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG141.0263929618768328No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT141.0263929618768328No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA120.8797653958944283No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG110.8064516129032258No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG100.7331378299120235No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT90.6598240469208211No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA90.6598240469208211No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA90.6598240469208211No Hit
TACCCTTACGATGTTCCTGATTGTGGGCTCGAAGATTTCGTTGGGGACTC90.6598240469208211No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC80.5865102639296188No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG80.5865102639296188No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG80.5865102639296188No Hit
TACCCTTACGATGTTCCTGATTTCGTTGGGGACTCTGCTCTTATTCGAAG70.5131964809384164No Hit
TACCCTTACGATGTTCCTGATTACGTTGGGGACTCTGCTCTTATTCGAAG70.5131964809384164No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG60.43988269794721413No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA60.43988269794721413No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG60.43988269794721413No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT60.43988269794721413No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG50.36656891495601174No Hit
TACCCTTACGATGTTCCTGATTACTGGGCTCGAAGATTTCGTTGGGGACT50.36656891495601174No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT50.36656891495601174No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGAAGATTTCGTTGGGGACTCT50.36656891495601174No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT50.36656891495601174No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCCTGATACGTGTCATTT50.36656891495601174No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC50.36656891495601174No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA40.2932551319648094No Hit
GATGAGTCGGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.2932551319648094No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG40.2932551319648094No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.2932551319648094No Hit
TACCCTTACGATGTTCCTGATTACGGTGTGGGCTCGAAGATTTCGTTGGG40.2932551319648094No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG40.2932551319648094No Hit
TACCCTTACGATGTTCCTGATTATGCTCGAAGATTTCGTTGGGGACTCTG40.2932551319648094No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT30.21994134897360706No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT30.21994134897360706No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT30.21994134897360706No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA30.21994134897360706No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTAGCATTT30.21994134897360706No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT30.21994134897360706No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGCTGGGGA30.21994134897360706No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTGCGTTGGGGACTCTG30.21994134897360706No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA30.21994134897360706No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT30.21994134897360706No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG30.21994134897360706No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGTATTT30.21994134897360706No Hit
TACCCTTACGCTGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA20.1466275659824047No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGGCTCGAAGATTTCGTTGGG20.1466275659824047No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCATTT20.1466275659824047No Hit
CTGTAAAGATAAATGGGTCCTCTAAAGAGTATGGAGCTGGGATTGACTCG20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCGCTTAGATTTCGGTGGGGA20.1466275659824047No Hit
TACCCTTACGATGTTCCTGAAGATTTCGTTGGGGACTCTGTCTCTTATAC20.1466275659824047No Hit
GATGCGTCCGCGTCTAACATGAACCCTTTATTTCATGATACGTGTCATTT20.1466275659824047No Hit
GATGAGTCAGCGTCGAACATGCAACATTTAATGCATGATACGTGGCATTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGG20.1466275659824047No Hit
GATGAGTCAGCGTCGAACATGCAACACTTATTGCATGATACGTGGCATTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGG20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGTCTCGAATATTTCGTTGT20.1466275659824047No Hit
TACCCTTACGCTGTTCCTGCTTACGCTCTACGATTTCGTTTGGGACTCTT20.1466275659824047No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCCTTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCTCGATGATTTCGTTGGGGACTCTG20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG20.1466275659824047No Hit
TACCCTTACGAGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTTG20.1466275659824047No Hit
GCTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATATAGTCCAGCCAGACGAGTCGTGGTAAAT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAATATTTCGTTGT20.1466275659824047No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGTCATTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCATCCGATTCGTGTTAAAT20.1466275659824047No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATGATACGTGGCATTT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAATATTTCGTTGG20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTATGCTCGACGATTTCGTTGGGGACTCTG20.1466275659824047No Hit
TACCCTTACGATGTTCCTGATTTCGTTGTGGACTCTGCTCTTATTCGAAG20.1466275659824047No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)