FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3A03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3A03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830
Sequences flagged as poor quality0
Sequence length301
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT26214.316939890710382No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCGCCTAA1779.672131147540984No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA1407.650273224043716No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA1146.229508196721312No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA1075.8469945355191255No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA1075.8469945355191255No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG633.4426229508196724No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA382.0765027322404372No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG341.8579234972677594No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAA301.639344262295082No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG281.530054644808743No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG221.2021857923497268No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT180.9836065573770493No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG160.8743169398907104No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG130.7103825136612022No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG120.6557377049180327No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT90.49180327868852464No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT90.49180327868852464No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG90.49180327868852464No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG80.4371584699453552No Hit
TACCCTTACGATGTTCCTGATTACGCTTGTGGGCTCGAAGATTTCGTTGG80.4371584699453552No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA80.4371584699453552No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG70.3825136612021858No Hit
TACCCTTACGATGTTCCTGATTACGCTTAAGCGGCCGCAAAAGGAAAATC70.3825136612021858No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCACATGCAGCCCGCCTAA70.3825136612021858No Hit
GATGAGTCCGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT60.32786885245901637No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA50.273224043715847No Hit
TACCCTTACGATGTTCCTGATGGGCTCGAAGATTTCGTTGGGGACTCTGT50.273224043715847No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCATCCCGCCTAA40.2185792349726776No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT40.2185792349726776No Hit
GATGAGTCAGCGTCGAACATGCACCATTTATTGCATGATACGTGGCATTT40.2185792349726776No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA40.2185792349726776No Hit
TACCCTTACGATGTTCCTGATTACGCACGATTTGCTGCCAGCCCGCCTAA40.2185792349726776No Hit
GATGCGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT40.2185792349726776No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA40.2185792349726776No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGCTCGAAGATTTCGTTGGGG40.2185792349726776No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT40.2185792349726776No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG40.2185792349726776No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGAAGATTTCGTTGGGGACTC40.2185792349726776No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG40.2185792349726776No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG40.2185792349726776No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA30.16393442622950818No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCATCCCGCCTAA30.16393442622950818No Hit
GATGAGTCAGCGTCGAACATTCAACATTTATTGCATGATACGTGGCATTT30.16393442622950818No Hit
TACCCTTACGATGTTCCTGATTACGCATTATTTGCTGCCAGCCCGCCTAA30.16393442622950818No Hit
TACCCTTACGATGTTCCTGATTACGCACAATGTGCAAACAGCCCGCCTAA30.16393442622950818No Hit
TACCCTTACGATGTCCTGATTACGCATGAGCCTGGAACAGCCCGCCTAAT30.16393442622950818No Hit
TACCCTTACGCTGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA30.16393442622950818No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA30.16393442622950818No Hit
TACCCTTACGATGTTCCTGATTACGCATGATTTGCTGCCATCCCGCCTAA30.16393442622950818No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGGAGATTTCGTTG30.16393442622950818No Hit
GATGAGTCAGCGTCGAACATGCGACATTTATTGCATGATACGTGGCATTT30.16393442622950818No Hit
GATGAGTCAGCGTCAAACATGCAACATTTATTGCATGATACGTGGCATTT30.16393442622950818No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG30.16393442622950818No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT30.16393442622950818No Hit
TACCCTTACGATGTTCCTGATTACGCGTTCGTGGGCTCGAAGATTTCGTT20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGATTTTGCCAGCCCGCCTAA20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACATCCCGCCTAA20.1092896174863388No Hit
GATGAGTCATCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1092896174863388No Hit
GATGAGTCAGCGTCTAACATGCAACATTTATTGCATAATACGTGGCATTT20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG20.1092896174863388No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATCGCATGATACGTGGCATTT20.1092896174863388No Hit
GATGAGTCAGCGTCGAGCATGCAACATTTATTGCATGATACGTGGCATTT20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTTCAAACAGCCCGCCTAA20.1092896174863388No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTGCATGATACGTGGCATTT20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAAATGCAGCCCTCCTAA20.1092896174863388No Hit
GATGAGTCAGCGTCGAACATGCAACATTTAATGCATGATACGTGGCATTT20.1092896174863388No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGTCATTT20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGA20.1092896174863388No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCGTTT20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGGTTTCGTTG20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCATAGAGCAAATGCAGCCCGCCTAA20.1092896174863388No Hit
GATGGGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1092896174863388No Hit
GATGTGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACATCCCGCCTAA20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGAATAATGTGCAAACAGCCCGCCTAA20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCATAGTGCAACTGCAGCCCGCCTAA20.1092896174863388No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCCTGATACGTGTCATTT20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG20.1092896174863388No Hit
GCTGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCCTGTGGGCTCGAAGATTTCGTTGG20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCATGAGCCTGGAACATCCCGCCTAA20.1092896174863388No Hit
GATGAGTCAGCGTAGAACATGCAACATTTATTGCATGATACGTGGCATTT20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGGTT20.1092896174863388No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTC20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCGTTGGT20.1092896174863388No Hit
TACCCTTACTATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA20.1092896174863388No Hit
GATACTAGTGGAGGAAGAAAAAATGGTCTTCACACTCGAAGATTTCGTTG20.1092896174863388No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTTCATGATACGTGGCATTT20.1092896174863388No Hit
TACCCTTACGCTGTTCCTGATTACGCATGATTTGCTGCCAGCCCGCCTAA20.1092896174863388No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTCA20.1092896174863388No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)