FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP3A02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP3A02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82
Sequences flagged as poor quality0
Sequence length301
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1214.634146341463413No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA89.75609756097561No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG56.097560975609756No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT44.878048780487805No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG44.878048780487805No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT33.6585365853658534No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG33.6585365853658534No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG22.4390243902439024No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG22.4390243902439024No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT22.4390243902439024No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTCAGATTTCGTTGGGGA11.2195121951219512No Hit
TACCATTACGATGTTCCTGATTACGCGTAAGCGGACGCAAGAGGAAAATG11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCGCGAGGATTTCGTTTTGGACTCTG11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGCTGGGGA11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGCAGATTTCGTTGGG11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTAGTTGCCAAAGCTGAACCCTTCTGTCTC11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGGGAGGTGGGAGCGAAGCTTTCGTTG11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGATGGT11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCTCATCGTGTTGGTTAAGGAATCTG11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCCGGGAGCTTTCATAGTTCGACGCT11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCATGGGCTCGAAGATTTCGTT11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTG11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGTCTCTGGGCTCGAAGATTTCGTTGG11.2195121951219512No Hit
GATGAGTCAGCGTCGAACATACAACATTTATTGCATGATACGTGGCATTT11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGGTGGGGA11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCTGAGCTCTCAGATTTCGTTGGGGA11.2195121951219512No Hit
GATGCGTCCGCGTCTAACATGCACCCTTTATTCCATGATACGTGGCATTT11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCGCAAATTCTTAGTCGAAGATTTCG11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACTCCCGGAAATTTTCTTTGGGACTCTT11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGTTGTCTCTACTATTTCGTTTGGGAC11.2195121951219512No Hit
TACCCTTACGCTGTTCCTGATTACGCGGCGGTCTCAGATGTCGTTGGGGA11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTAAGCTCTACGAGTTAGGTTGGGGCTCTT11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGTCTCAGAGTTCGCGGGGGA11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTCGGCTCTATGATTTCGTTG11.2195121951219512No Hit
CTGTAACGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTTG11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGGGTCCGTTGGCTCGAAGATTTCGTT11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTATCAGATTTCGTTGGGAC11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGATCGAAACTTTCGGTT11.2195121951219512No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGACCTGTCT11.2195121951219512No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)