FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP2H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP2H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12788
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG601046.997184860807No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG5754.496402877697841No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA3963.0966531122927745No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT2091.63434469815452No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTGGTGTTAAAGGAAGGCG1881.4701282452299032No Hit
TACCCTTACGATGTTCCTGATTAGTTGACAAAGCTGAACCCTTCTGTCTC1831.4310290897716609No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG1781.3919299343134188No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG1731.3528307788551768No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTGTTGACAAAGCTGAACCCTTC1621.2668126368470443No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1291.0087582108226463No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG1160.9071004066312168No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG1040.8132624335314358No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG920.7194244604316548No Hit
TACCCTTACGATGTTCCTGATGTTGACAAAGCTGAACCCTTCTGTCTCTT880.688145136065061No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT770.6021269940569284No Hit
TACCCTTACGATGTTCCTGATTACGTTGACAAAGCTGAACCCTTCTGTCT760.59430716296528No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT760.59430716296528No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG700.5473881764153895No Hit
CTGTAAAGCTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG650.5082890209571473No Hit
TACCCTTACGATGTTCCTGACAAAGCTGAACCCTTCTGTCTCTTATACAC620.4848295276822021No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT610.47700969659055364No Hit
TACCCTTACGATGTTCCTGATTACGATTGACAAAGCTGAACCCTTCTGTC500.390991554582421No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG490.3831717234907726No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT460.3597122302158274No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGGCCGCTGAGGGCAGAGGGAA420.3284329058492337No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA390.3049734125742884No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG360.28151391929934316No Hit
CTGTCAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG310.24241476384110106No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG290.2267751016578042No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG280.21895527056615577No Hit
TACCCTTACGATGTTCCTGATTACGCTCGTCGGCAGCGTCAGATGTTGAC270.21113543947450736No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA270.21113543947450736No Hit
TACCCTTACGATGTTCCTGACAACGCTGAACCCTTCTGTCTCTTATACAC260.20331560838285895No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG250.1954957772912105No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG250.1954957772912105No Hit
CTGTAAAGATACATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG250.1954957772912105No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC240.1876759461995621No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT240.1876759461995621No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG240.1876759461995621No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT220.17203628401626525No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG210.16421645292461684No Hit
CTGTAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCGG210.16421645292461684No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA210.16421645292461684No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG200.15639662183296843No Hit
CTGTAAAGATAAATGGGTCATCTAAAGTGTATGGAGCTGGGATTGACTCG200.15639662183296843No Hit
TACCCTTACGATGTTCCTGATTCATTGACAAAGCTGAACCCTTCTGTCTC200.15639662183296843No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG190.14857679074132No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG170.13293712855802314No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTG160.12511729746637473No Hit
TACCCTTACGATGTTCCTGTTGACAAAGCTGAACCCTTCTGTCTCTTATA160.12511729746637473No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC150.11729746637472631No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT150.11729746637472631No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGAAGATTTCGTTGGGGA150.11729746637472631No Hit
CTGTACAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG140.10947763528307788No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGATCTGT140.10947763528307788No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTTTTGACAAAGCTGAACCCTTC140.10947763528307788No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGCTCGAAGATTTCGTTGG140.10947763528307788No Hit
TACCCTTACGATGTTCCTGATTCGGGGCTCGAAGATTTCGTTGGGGACTC140.10947763528307788No Hit
TACCCTTACGATGTTCCTGATTACGCTTAAGCGGCCGCAAAAGGAAAATC130.10165780419142947No Hit
CTGTAAAGATAAATGTGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG130.10165780419142947No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT130.10165780419142947No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG130.10165780419142947No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG130.10165780419142947No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)