FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP2G09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP2G09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9979
Sequences flagged as poor quality0
Sequence length301
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG491849.28349534021445No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG4464.4693857099909815No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA3523.5274075558673212No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT1471.473093496342319No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG1451.453051407956709No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG1341.3428199218358554No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT1251.2526305241006113No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTGGTGTTAAAGGAAGGCG880.8818518889668303No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT870.8718308447740254No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTGTTGACAAAGCTGAACCCTTC860.8618098005812206No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG860.8618098005812206No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG810.8117045796171961No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT800.8016835354243912No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG770.7716204028459765No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG640.641346828339513No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG590.5912416073754885No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG480.48101012125463477No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT450.4509469886762201No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT440.44092594448341516No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC430.4309049002906103No Hit
CTGTAAAGCTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG400.4008417677121956No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG310.3106523699769516No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG290.29061028159134183No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG270.27056819320573205No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG260.26054714901292714No Hit
CTGTAAAGATAAATGGGTCATCTAAAGTGTATGGAGCTGGGATTGACTCG240.24050506062731739No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG230.23048401643451247No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA220.22046297224170758No Hit
TACCCTTACGATGTTCCTGATTGACAAAGCTGAACCCTTCTGTCTCTTAT220.22046297224170758No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG210.21044192804890272No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT210.21044192804890272No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG200.2004208838560978No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG200.2004208838560978No Hit
CTGTACAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG190.19039983966329294No Hit
CTGTAAAGATACATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG190.19039983966329294No Hit
TACCCTTACGATGTTCCTGATTAGTTGACAAAGCTGAACCCTTCTGTCTC180.18037879547048802No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA180.18037879547048802No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG170.17035775127768313No Hit
CTGTAAAGATAAATGGGTCATCTGGGGTTGACAAAGCTGAACCCTTCTGT160.16033670708487824No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG160.16033670708487824No Hit
TACCCTTACGATGTTCCTGATTACGCGTAAATTCCTAGTCGAAGATTTCG150.15031566289207335No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG130.13027357450646357No Hit
CTGTAAAGATAAATGTGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG130.13027357450646357No Hit
CTGTAAAGATAACTGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG130.13027357450646357No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA120.12025253031365869No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGCGCTGGGATTGACTCG120.12025253031365869No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTCTGGAGCTGGGATTGACTCG110.11023148612085379No Hit
CTGTAAAGATAAATGGGTCATCTAACGAGTATGGAGCTGGGATTGACTCG110.11023148612085379No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCGAAGATTTCGTTGGG110.11023148612085379No Hit
CTGTAAAGATAAATGGGACATCTAAAGAGTATGGAGCTGGGATTGACTCG110.11023148612085379No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGGAGATTTCGTTG110.11023148612085379No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT110.11023148612085379No Hit
CTGTAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCGG110.11023148612085379No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTTTTGACAAAGCTGAACCCTTC110.11023148612085379No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT110.11023148612085379No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT110.11023148612085379No Hit
CTGTAAAGATAAATGGGTCATCTCAAGAGTATGGAGCTGGGATTGACTCG100.1002104419280489No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT100.1002104419280489No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT100.1002104419280489No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG100.1002104419280489No Hit
CTGTAACGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG100.1002104419280489No Hit
CTGTAAAGATAAATGGGTCCTCTAAAGAGTATGGAGCTGGGATTGACTCG100.1002104419280489No Hit
TACCCTTACGATGTTCCTGATTACGCTTCGTGGCTCGAAGATTTCGTTGG100.1002104419280489No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)