FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP2G06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP2G06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24144
Sequences flagged as poor quality0
Sequence length301
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1625767.33349900596421No Hit
TACCCTTACGATGTTCCTGATTACGCATAATTCAGTGCCAGCCCGCCTAA3891.611166335321405No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG3581.4827700463883366No Hit
TACCCTTACGATGTTCCTGATTACGCATAATGTGCAAACAGCCCGCCTAA3521.4579191517561298No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA3371.3957919151756129No Hit
TACCCTTACGATGTTCCTGATTACGCATAAGAGTTTGCCAGCCCGCCTAA3351.387508283631544No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG2561.0603048376408217No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG1980.8200795228628232No Hit
CTGTAAAGCTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1590.6585487077534791No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT1500.621272365805169No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG1200.4970178926441352No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG890.3686216037110669No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG880.36447978793903246No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG840.34791252485089463No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT770.3189198144466534No Hit
CTGTACAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG760.3147779986746189No Hit
CTGTAAAGATAAATGGGTCATCTAAAGTGTATGGAGCTGGGATTGACTCG690.28578528827037775No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC680.28164347249834326No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG630.26093439363817095No Hit
CTGTAAAGATACATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG610.2526507620941021No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG560.23194168323392977No Hit
CTGTAAAGATAAATGGGACATCTAAAGAGTATGGAGCTGGGATTGACTCG530.21951623591782635No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT520.21537442014579194No Hit
TACCCTTACGATGTTCCTGATTAGTTGACAAAGCTGAACCCTTCTGTCTC520.21537442014579194No Hit
CTGTAAAGATAACTGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG470.1946653412856196No Hit
CTGTAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCGG430.17809807819748177No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT430.17809807819748177No Hit
ATGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG420.17395626242544732No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTCTGGAGCTGGGATTGACTCG390.16153081510934394No Hit
CTGTAAAGATAAATGGGTCATCTAACGAGTATGGAGCTGGGATTGACTCG390.16153081510934394No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGCGCTGGGATTGACTCG390.16153081510934394No Hit
CTGTAAAGATAAATGGGTCCTCTAAAGAGTATGGAGCTGGGATTGACTCG380.15738899933730946No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG370.15324718356527503No Hit
CTGTAAAGATAAATGGGTCATCTACAGAGTATGGAGCTGGGATTGACTCG350.14496355202120612No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGCTTGACTCG350.14496355202120612No Hit
CTATAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG340.14082173624917163No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT340.14082173624917163No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG340.14082173624917163No Hit
CTGTAAAGATAAATGTGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG320.13253810470510272No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA310.12839628893306826No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT290.12011265738899933No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTGGTGTTAAAGGAAGGCG290.12011265738899933No Hit
CTGTAACGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG270.11182902584493042No Hit
CTGTAAAGATAAATGGGTCATCTAAAGCGTATGGAGCTGGGATTGACTCG270.11182902584493042No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT260.10768721007289597No Hit
CTGTCAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG260.10768721007289597No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA250.1035453943008615No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)