FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP2E08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP2E08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11734
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG181115.43378217146753No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG9387.993863985000853No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA6955.922958922788478No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG4423.766831430032384No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTGGTGTTAAAGGAAGGCG3512.9913073120845404No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG2852.428839270495995No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT2752.3436168399522757No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT2752.3436168399522757No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT1431.2186807567751832No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG1181.0056246804158855No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT1120.954491222089654No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG1090.9289244929265382No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC1040.8863132776546788No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGGCCGCTGAGGGCAGAGGAAG990.8437020623828192No Hit
TACCCTTACGATGTTCCTGATTGTTGACAAAGCTGAACCCTTCTGTCTCT970.8266575762740754No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG840.7158684165672404No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG790.6732572012953809No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA750.6391682290778933No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT710.6050792568604056No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG710.6050792568604056No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG670.570990284642918No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT660.5624680415885461No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT630.5369013124254304No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG580.4942900971535708No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT510.4346343957729674No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA480.4090676666098517No Hit
TACCCTTACGATGTTCCTGATTACGGTTGACAAAGCTGAACCCTTCTGTC470.40054542355547984No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG450.38350093744673597No Hit
TACCCTTACGATGTTCCTGATTACGTTGGGGACTCTGCTCTTATTCGAAG420.3579342082836202No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG410.34941196522924833No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA400.34088972217487645No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG390.3323674791205045No Hit
TACCCTTACGATGTTCCTGATTACGCTTGTGACAAAGCTGAACCCTTCTG380.3238452360661326No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG370.3153229930117607No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG370.3153229930117607No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT370.3153229930117607No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT370.3153229930117607No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT360.30680074995738876No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTGGTGTTAACGGAAGGCG350.2982785069030169No Hit
TACCCTTACGATGTTCCTGATTACATATGTGACAAAGCTGAACCCTTCTG340.28975626384864495No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT330.28123402079427307No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC330.28123402079427307No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG320.27271177773990113No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG320.27271177773990113No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG320.27271177773990113No Hit
CTGTAAAGATAAATGGGTCATCACACTCGAAGATTTCGTTGGGGACTCTG290.2471450485767854No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG260.2215783194136697No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG260.2215783194136697No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT260.2215783194136697No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG240.20453383330492586No Hit
TACCCTTACGATGTTCCTGATGACAAAGCTGAACCCTTCTGTCTCTTATA220.18748934719618204No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG210.1789671041418101No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT200.17044486108743823No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGT200.17044486108743823No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA200.17044486108743823No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG200.17044486108743823No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT190.1619226180330663No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCGGGCTCGAAGATTTCGTTGG190.1619226180330663No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT190.1619226180330663No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGCAGATTTCGTTGGGGA180.15340037497869438No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA180.15340037497869438No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTTGTGTTAACGGACGGCT180.15340037497869438No Hit
CTGTAAAGCTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG170.14487813192432247No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA170.14487813192432247No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA170.14487813192432247No Hit
TACCCTTACGATGTTCCTGATTACGCTCTTTCCCTACTCGAAGATTTCGT170.14487813192432247No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTG160.13635588886995056No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG150.12783364581557866No Hit
TACCCTTACGATGTTCCTGATCTTGGGCTCGAAGATTTCGTTGGGGACTC150.12783364581557866No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGAAGATTTCGTTGGGGA150.12783364581557866No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTT140.11931140276120675No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG140.11931140276120675No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT140.11931140276120675No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCTGGGCTCGAAGATTTCGTTG140.11931140276120675No Hit
TACCCTTACGATGTTCCTGATTCGGGGCTCGAAGATTTCGTTGGGGACTC140.11931140276120675No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT130.11078915970683485No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT130.11078915970683485No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG130.11078915970683485No Hit
TACCCTTACGATGTTCCTGATTACTGGGCTCGAAGATTTCGTTGGGGACT120.10226691665246293No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)