FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP2E04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP2E04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21839
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1392663.76665598241678No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG7233.3105911442831633No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA4832.2116397270937314No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG3131.4332158065845506No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG2511.149320023810614No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT2241.025687989376803No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG2010.9203718118961491No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTGGTGTTAAAGGAAGGCG1780.8150556344154952No Hit
CTGTAAAGCTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1450.6639498145519483No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG1420.6502129218370805No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT1080.4945281377352443No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG1070.48994917349695505No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG890.4075278172077476No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG840.38463299601630113No Hit
CTGTAAAGATACATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG680.31136956820367234No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC670.306790603965383No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTGTTGACAAAGCTGAACCCTTC650.29763267548880445No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG640.29305371125051516No Hit
TACCCTTACGATGTTCCTGACAAAGCTGAACCCTTCTGTCTCTTATACAC620.2838957827739365No Hit
TACCCTTACGATGTTCCTGTTGACAAAGCTGAACCCTTCTGTCTCTTATA600.2747378542973579No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG460.21063235496130775No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT450.20605339072301845No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG450.20605339072301845No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG440.20147442648472913No Hit
CTGTAAAGATAAATGGGTCATCTAAAGTGTATGGAGCTGGGATTGACTCG420.19231649800815057No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT380.17400064105499335No Hit
TACCCTTACGATGTTCCTGATTAGTTGACAAAGCTGAACCCTTCTGTCTC370.16942167681670406No Hit
CTGTAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCGG360.16484271257841476No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT330.15110581986354685No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA330.15110581986354685No Hit
CTGTAAAGATAAATGGGTCATCTAACGAGTATGGAGCTGGGATTGACTCG310.14194789138696826No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT300.13736892714867896No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG290.1327899629103897No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG280.12821099867210037No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA280.12821099867210037No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGCGCTGGGATTGACTCG280.12821099867210037No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT270.12363203443381107No Hit
TACCCTTACGATGTTCCTGACAACGCTGAACCCTTCTGTCTCTTATACAC260.11905307019552176No Hit
CTGTAAAGATAACTGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG260.11905307019552176No Hit
CTGTACAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG240.10989514171894318No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG240.10989514171894318No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG240.10989514171894318No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT240.10989514171894318No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA240.10989514171894318No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG230.10531617748065387No Hit
CTGTAACGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG230.10531617748065387No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTCTGGAGCTGGGATTGACTCG220.10073721324236456No Hit
CTGTAAAGATAAATGGGTCATCTAAAGCGTATGGAGCTGGGATTGACTCG220.10073721324236456No Hit
CTGTAAAGATAAATGGGTCCTCTAAAGAGTATGGAGCTGGGATTGACTCG220.10073721324236456No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)