FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP2E02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP2E02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6558
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG66710.170783775541324No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA4847.380298871607198No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG3946.007929246721561No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG3074.68130527599878No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT2854.34583714547118No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTGTTGACAAAGCTGAACCCTTC1612.4550167734065265No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG1412.150045745654163No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG1161.7688319609637084No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT1001.5248551387618177No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT941.4333638304361085No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG901.3723696248856359No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG831.2656297651723085No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGGCCGCTGAGGGAGAGGAATT761.1588899054589814No Hit
TACCCTTACGATGTTCCTGATTACGCAGTTGACAAAGCTGAACCCTTCTG741.128392802683745No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG640.9759072888075633No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG590.8996645318694725No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT530.8081732235437634No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC510.777676120768527No Hit
TACCCTTACGATGTTCCTGACAAAGCTGAACCCTTCTGTCTCTTATACAC510.777676120768527No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT470.7166819152180542No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT370.5641964013418725No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCGGGCTCGAAGATTTCGTTGG350.5336992985666361No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG330.5032021957913998No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT310.4727050930161634No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT290.4422079902409271No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA290.4422079902409271No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG280.4269594388533089No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG280.4269594388533089No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA270.41171088746569073No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG230.35071668191521804No Hit
TACCCTTACGATGTTCCTGTTGACAAAGCTGAACCCTTCTGTCTCTTATA230.35071668191521804No Hit
TACCCTTACGATGTTCCTGACAACGCTGAACCCTTCTGTCTCTTATACAC230.35071668191521804No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC230.35071668191521804No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG210.32021957913998167No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG210.32021957913998167No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG210.32021957913998167No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTTTTGACAAAGCTGAACCCTTC210.32021957913998167No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG200.3049710277523635No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT190.28972247636474535No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG190.28972247636474535No Hit
TACCCTTACGATGTTCCTGATTACGTCGTCGGCAGCGTCAGATGTTGACA190.28972247636474535No Hit
TACCCTTACGATGTTCCTGATTACGCGTAAATTCCTAGTCGAAGATTTCG190.28972247636474535No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCTAATATTTCTTTGGT180.2744739249771272No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG180.2744739249771272No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGG180.2744739249771272No Hit
TACCCTTACGATGTTCCTGATTACAAAGCTGAACCCTTCTGTCTCTTATA160.24397682220189082No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT160.24397682220189082No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG160.24397682220189082No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG150.22872827081427266No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGGGCTCGAAGATTTCGTTGG150.22872827081427266No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGCTCGAAGATTTCGTTG140.21347971942665445No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG140.21347971942665445No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT140.21347971942665445No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGAGCTCGAAGATTTCGTTGGG140.21347971942665445No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGGCTCGAAGATTTCGTTG140.21347971942665445No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT130.19823116803903632No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA130.19823116803903632No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT130.19823116803903632No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG130.19823116803903632No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT130.19823116803903632No Hit
TACCCTTACGATGTTCCTGATTACGCTTAAGCGGCCGCAAAAGGAAAATC120.18298261665141813No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGCAGATTTCGTTGGGGA120.18298261665141813No Hit
TACCCTTACGATGTTCCTGATTACGCTTGTGACAAAGCTGAACCCTTCTG120.18298261665141813No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT110.16773406526379994No Hit
TACCCTTACGATGTTCCTGATTACGCGTGTGCTCGAAGATTTCGTTGGGG110.16773406526379994No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGCTCGAAGATTTCGTTGGGT110.16773406526379994No Hit
TACCCTTACGATGTTCCTGATTACGCTTTACGATTTCGTTTGGGACTCTT100.15248551387618176No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT100.15248551387618176No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTGATGACAAAGCTGAACCCTTC100.15248551387618176No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG100.15248551387618176No Hit
TACCCTTACGATGTTCCTGATTACGCGTTGGGCTCGAAGATTTCGTTGGG90.1372369624885636No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTAAGATTTCGTTGGGGA90.1372369624885636No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG90.1372369624885636No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA90.1372369624885636No Hit
GATGAGTCAGCGTCGAACATGCAACATCTATTGCATGATACGTGGCATTT90.1372369624885636No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGTCGAAGATTTCGTTG70.10673985971332722No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTAGTGGGCTCGAAGATTTCGT70.10673985971332722No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGCTTTCGTTGGGGACTCTG70.10673985971332722No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGCTCGAAGATTTCGTTGGGT70.10673985971332722No Hit
TACCCTTACGATGTTCCTGATTACGCCTGGCTCGAAGATTTCGTTGGGGA70.10673985971332722No Hit
TACCCTTACGATGTTCCTGATTACGCGTTCGTGGGCTCGAAGATTTCGTT70.10673985971332722No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTTTTGACACAGCTGAACCCTTC70.10673985971332722No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT70.10673985971332722No Hit
TACCCTTACGATGTTCCTGATTACGCCTTGGCTCGAAGATTTCGTTGGGG70.10673985971332722No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGACTCGAAGATTTCGTTGGGG70.10673985971332722No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)