FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP2D09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP2D09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3778
Sequences flagged as poor quality0
Sequence length301
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG38810.26998411858126No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA2867.57014293276866No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG1784.71148755955532No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTGTTGACAAAGCTGAACCCTTC1534.049761778718898No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT1524.023292747485442No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG1463.8644785600847014No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG1463.8644785600847014No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG671.7734250926416093No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG651.7204870301746955No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG601.5881418740074114No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG541.4293276866066702No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG501.3234515616728428No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC481.270513499205929No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT451.1911064055055587No Hit
TACCCTTACGATGTTCCTGATTAGTTGACAAAGCTGAACCCTTCTGTCTC421.1116993118051879No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGGCTCGAAGATTTCGTT330.8734780307040761No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT320.8470089994706195No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGCTCGAAGATTTCGTTGG300.7940709370037057No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG250.6617257808364214No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG240.6352567496029645No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA230.6087877183695076No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT210.5558496559025939No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGGGGCTCGAAGATTTCGTTGG200.5293806246691372No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG190.5029115934356803No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA190.5029115934356803No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTTTTGACAAAGCTGAACCCTTC180.4764425622022234No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT180.4764425622022234No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGGTAAAT170.4499735309687666No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG150.39703546850185284No Hit
TACCCTTACGATGTTCCTGATTACGCGGCTCGAAGATTTCGTTGGGGACT140.37056643726839594No Hit
TACCCTTACGATGTTCCTGATTACGGTTCGTGGCTCGAAGATTTCGTTGG140.37056643726839594No Hit
CTGTAAAGATAAATGGGTCATCTCGAAGATTTCGTTGGGGACTCTGTCTC130.3440974060349391No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGGGCTCGAAGATTTCGTTGGG130.3440974060349391No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTT120.31762837480148226No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGGTG120.31762837480148226No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGTGGGCTCGAAGATTTCGTTG110.2911593435680254No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCTGGGCTCGAAGATTTCGTTG110.2911593435680254No Hit
TACCCTTACGATGTTCCTGATTATGTTCCTGATTACGCTTGGGCTCGAAG110.2911593435680254No Hit
TACCCTTACGATGTTCCTGATTACGCTCTACGATTTCGTTTGGGACTCTT100.2646903123345686No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTGGGGACTCTG100.2646903123345686No Hit
TACCCTTACGATGTTCCTGATTCGGGGCTCGAAGATTTCGTTGGGGACTC100.2646903123345686No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGG90.2382212811011117No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTT80.21175224986765487No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTGGGGACTCTG80.21175224986765487No Hit
TACCCTTACGATGTTCCTGATTACGCTCCGGGCTCGAAGATTTCGTTGGG80.21175224986765487No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGGGCTCGAAGATTTCGTTG80.21175224986765487No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGAGCTCGAAGATTTCGTTGGG70.18528321863419797No Hit
TACCCTTACGATGTTCCTGATTACGCTCTCGTGGGCTCGAAGATTTCGTT70.18528321863419797No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGGGCTCGAAGATTTCGTTGGG70.18528321863419797No Hit
TACCCTTACGATGTTCCTGATTACGCTCTGTGGGCTCGAAGATTTCGTTG70.18528321863419797No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCTGGGCTCGAAGATTTCGTTG70.18528321863419797No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGGAGATTTCGTTG70.18528321863419797No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCTAAGATTTCGTTGGGGA60.15881418740074113No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTGATGACAAAGCTGAACCCTTC60.15881418740074113No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGCTCGAAGATTTCGTT60.15881418740074113No Hit
TACCCTTACGATGTTCCTGATTACGCCTGTGGGCTCGAAGATTTCGTTGG60.15881418740074113No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGT60.15881418740074113No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGT60.15881418740074113No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGATCGAAGATTTCGTTGGGGA60.15881418740074113No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAATATTTCGTTGGG60.15881418740074113No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTT60.15881418740074113No Hit
TACCCTTACGATGTTCCTGATTACGCTTTGGCTCGAAGATTTCGTTGGGT60.15881418740074113No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG60.15881418740074113No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCTCAGATTTCGTTGGGGA50.1323451561672843No Hit
TACCCTTACGATGTTCCTGATTACGGTCCGTGGGCTCGAAGATTTCGTTG50.1323451561672843No Hit
TACCCTTACGATGTTCCTGATTACGCGTTCGTGGGCTCGAAGATTTCGTT50.1323451561672843No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGT50.1323451561672843No Hit
TACCCTTACGATGTTCCTGATTACGTTGGGGACTCTGCTCTTATTCGAAG50.1323451561672843No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAAGATTTCGTTGGGGACTCTG50.1323451561672843No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCGTGGGCTCGAAGATTTCGT50.1323451561672843No Hit
TACCCTTACGATGTTCCTGATTACGGGGCTCGAAGATTTCGTTGGGGACT50.1323451561672843No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCATACGATTCGTGTTAAAT50.1323451561672843No Hit
TACCCTTACGATGTTCCTGATATCGTCTAGCCAGACGAGTCGTGTTAAAT50.1323451561672843No Hit
TACCCTTACGATGTTCCTGATTACGCGTAAATTCCTAGTCGAAGATTTCG50.1323451561672843No Hit
TACCCTTACGATGTTCCTGATTACGCCTGGCTCGAAGATTTCGTTGGGGA40.10587612493382743No Hit
TACCCTTACGATGTTCCTGATTACGTGGGCTCGAAGATTTCGTTGGGGAC40.10587612493382743No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTT40.10587612493382743No Hit
TACCCTTACGATGTTCCTGATTACGGTCTCGGGCTCGAAGATTTCGTTGG40.10587612493382743No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGT40.10587612493382743No Hit
TACCCTTACGATGTTCCTGATTACGATCGAAGATTTCGTTGGGGACTCTG40.10587612493382743No Hit
TACCCTTACGATGTTCCTGATTACGCTCTAAGATTTCGTTTGGGACTCTG40.10587612493382743No Hit
TACCCTTACGATGTTCCTGATTACGCTCGACGATTTCGTTTGGGACTCTG40.10587612493382743No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGCTTTCGTTG40.10587612493382743No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGACTCGAAGATTTCGTTGGGG40.10587612493382743No Hit
TACCCTTACGATGTTCCTGATTACGGTCGGCTCGAAGATTTCGTTGGGGA40.10587612493382743No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)