FastQCFastQC Report
Tue 7 Nov 2023
000000000-L65MN_l01_n01_PIELP2B09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-L65MN_l01_n01_PIELP2B09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17222
Sequences flagged as poor quality0
Sequence length301
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG1156767.16409243990245No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTGGGGACTCTG4002.3226106143305074No Hit
TACCCTTACGATGTTCCTGATTACGCTGGGCTCGAAGATTTCGTTGGGGA3351.9451863895018No Hit
CTGTAAAGGTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG2641.5329230054581349No Hit
TACCCTTACGATGTTCCTGATTACGCCGTGGGCTCGAAGATTTCGTTGGG1951.1322726744861225No Hit
TACCCTTACGATGTTCCTGATTACGCGTGGGCTCGAAGATTTCGTTGGGG1550.9000116130530718No Hit
TACCCTTACGATGTTCCTGATATAGTCTAGCCAGACGAGTCGTGGTAAAT1470.8535594007664614No Hit
GATGAGTCAGCGTCGAACATGCAACATTTATTGCATGATACGTGGCATTT1420.82452676808733No Hit
CTGTAAAGCTAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG990.5748461270468006No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGCTCGAAGATTTCGTTG920.5342004412960167No Hit
TACCCTTACGATGTTCCTGATTACGCTTGGGCTCGAAGATTTCGTTGGGG850.49355475554523287No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTGTGGGCTCGAAGATTTCGTT670.38903727790035997No Hit
TACCCTTACGATGTTCCTGATTACGCGTCGTGGCTCGAAGATTTCGTTGG670.38903727790035997No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTCTGGGCTCGAAGATTTCGTT520.301939379862966No Hit
CTGTAAAGATAAATGGGTCATCTAAAGTGTATGGAGCTGGGATTGACTCG510.29613285332713973No Hit
CTGTAAAGATACATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG510.29613285332713973No Hit
CTGTAAAGATAAATGGGTCATCTAATCTTGTTGACAAAGCTGAACCCTTC490.2845198002554872No Hit
TACCCTTACGATGTTCCTGATTACGGTCTGTGGGCTCGAAGATTTCGTTG490.2845198002554872No Hit
CTGTAAAGATAAATGGGCCATCTAAAGAGTATGGAGCTGGGATTGACTCG460.26710022064800837No Hit
TACCCTTACGATGTTCCTGATTACGCTGCGGCCGCTGAGGGCAGAGGAAG450.26129369411218206No Hit
TACCCTTACGATGTTCCTGATTACGCGTCTTGGGCTCGAAGATTTCGTTG440.2554871675763558No Hit
TACCCTTACGATGTTCCTGATTAGTTGACAAAGCTGAACCCTTCTGTCTC420.24387411450470328No Hit
CTGTAAAGATAAATGGGTCATCACACTCGAAGATTTCGTTGGGGACTCTG410.23806758796887703No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGAAGATTTCGTTGGGGACTCT400.23226106143305075No Hit
TACCCTTACGATGTTCCTGATTACGCTTGAATCCTCCTGATCTCCACCTT330.19161537568226686No Hit
CTGTCAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG320.1858088491464406No Hit
TACCCTTACGATGTTCCTGATTACGCCTCGTGGGCTCGAAGATTTCGTTG310.18000232261061433No Hit
TACCCTTACGATGTTCCTGATTACGATTGACAAAGCTGAACCCTTCTGTC290.16838926953896177No Hit
TACCCTTACGATGTTCCTGATTACGCTGTGCTCGAAGATTTCGTTGGGGA280.16258274300313552No Hit
TACCCTTACGATGTTCCTGATTACATATGTGACAAAGCTGAACCCTTCTG270.15677621646730924No Hit
CTGTAAAGATAAATGTGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG260.150969689931483No Hit
CTGTAAAGATAAATGGGTCATCTAACGAGTATGGAGCTGGGATTGACTCG230.13355011032400418No Hit
TACCCTTACGATGTTCCTGATTACGCTCGGGCTCGAAGATTTCGTTGGGG230.13355011032400418No Hit
TACCCTTACGATGTTCCTGATTACGCGGGCTCGAAGATTTCGTTGGGGAC220.1277435837881779No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTCTGGAGCTGGGATTGACTCG220.1277435837881779No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGCTTGACTCG220.1277435837881779No Hit
TACCCTTACGATGTTCCTGATTACGCGCTGTGGGCTCGAAGATTTCGTTG220.1277435837881779No Hit
TACCCTTACGATGTTCCTGATTACGCGTCCGTGGGCTCGAAGATTTCGTT220.1277435837881779No Hit
CTGTAAAGATAACTGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG220.1277435837881779No Hit
TACCCTTACGATGTTCCTGTTGACAAAGCTGAACCCTTCTGTCTCTTATA210.12193705725235164No Hit
CTGTACAGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG200.11613053071652538No Hit
CTGTAACGATAAATGGGTCATCTAAAGAGTATGGAGCTGGGATTGACTCG200.11613053071652538No Hit
TACCCTTACGATGTTCCTGATTACGGTGGGCTCGAAGATTTCGTTGGGGA200.11613053071652538No Hit
CTGTAAAGATAAATGGGTCATCTAAAGAGTATGGCGCTGGGATTGACTCG190.11032400418069911No Hit
TACCCTTACGATGTTCCTGATTACGCTTATCGTGGTGTTAAAGGAAGGCG180.10451747764487283No Hit
TACCCTTACGATGTTCCTGATTACGCTCGAAGATTTCGTTTGGGACTCTG180.10451747764487283No Hit
TACCCTTACGATGTTCCTGATTACGCGCTCGGGGCTCGAAGATTTCGTTG180.10451747764487283No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)